| 产品名称： | NCCIT |
|---|---|
| 商品货号： | TS211395 |
| Organism： | Homo sapiens, human |
| Tissue： | anterior mediastinal mixed germ cell tumor |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pluripotent embryonal carcinoma; teratocarcinoma |
| Age： | adult |
| Gender： | male |
| Ethnicity： | Japanese |
| Images： |  |
| Derivation： | NCCIT was established by Shinichi Teshima (National Cancer Institute, Tokyo, Japan) in 1985 from a mediastinal mixed germ cell tumor. |
| Clinical Data： | adult Japanese male |
| Tumorigenic： | Yes |
| Effects： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | This pluripotent stem cell line is capable of somatic and extraembryonic differentiation.
The undifferentiated cells are equivalent to a stage intermediate between seminoma and embryonal carcinoma.
They will differentiate in response to retinoic acid.
NCCIT cells are negative for keratin.
They are positive for vimentin and placental alkaline phosphatase.
The line was contaminated with mycoplasma, but was cured by the depositor prior to deposit at the ATCC. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture two times weekly. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Add fresh medium at the time of subculture |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 10,13 D7S820: 10 THO1: 7,9 TPOX: 8 vWA: 14,18 |
| Name of Depositor： | I Damjanov |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1985 |
| References： | Damjanov I, et al. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. Lab. Invest. 68: 220-232, 1993. PubMed: 7680083 Teshima S, et al. Four new human germ cell tumor cell lines. Lab. Invest. 59: 328-336, 1988. PubMed: 2842544 |
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| 产品名称： | NCCIT |
|---|---|
| 商品货号： | TS211395 |
| Organism： | Homo sapiens, human |
| Tissue： | anterior mediastinal mixed germ cell tumor |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pluripotent embryonal carcinoma; teratocarcinoma |
| Age： | adult |
| Gender： | male |
| Ethnicity： | Japanese |
| Images： | |
| Derivation： | NCCIT was established by Shinichi Teshima (National Cancer Institute, Tokyo, Japan) in 1985 from a mediastinal mixed germ cell tumor. |
| Clinical Data： | adult Japanese male |
| Tumorigenic： | Yes |
| Effects： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments： | This pluripotent stem cell line is capable of somatic and extraembryonic differentiation.
The undifferentiated cells are equivalent to a stage intermediate between seminoma and embryonal carcinoma.
They will differentiate in response to retinoic acid.
NCCIT cells are negative for keratin.
They are positive for vimentin and placental alkaline phosphatase.
The line was contaminated with mycoplasma, but was cured by the depositor prior to deposit at the ATCC. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37°C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture two times weekly. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Add fresh medium at the time of subculture |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 10,13 D7S820: 10 THO1: 7,9 TPOX: 8 vWA: 14,18 |
| Name of Depositor： | I Damjanov |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1985 |
| References： | Damjanov I, et al. Retinoic acid-induced differentiation of the developmentally pluripotent human germ cell tumor-derived cell line, NCCIT. Lab. Invest. 68: 220-232, 1993. PubMed: 7680083 Teshima S, et al. Four new human germ cell tumor cell lines. Lab. Invest. 59: 328-336, 1988. PubMed: 2842544 |
