| 产品名称： | MYC 1-9E10.2 9E10 |
|---|---|
| 商品货号： | TS211399 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | Spleen |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c (B cell); BALB/c (myeloma) |
| Applications： | The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc. |
| Derivation： | Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human myc (c-myc) protein |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human myc (c-myc) protein |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal:xa0Every 2 to 3 days |
| Isotype： | IgG1 |
| Name of Depositor： | J Bishop, GI Evan |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782 Van Ewijk WV, et al. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA 94: 3903-3908, 1997. PubMed: 9108077 Cheng M, et al. ERK3 is a constitutively nuclear protein kinase. J. Biol. Chem. 271: 8951-8958, 1996. PubMed: 8621539 |
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| 产品名称： | MYC 1-9E10.2 9E10 |
|---|---|
| 商品货号： | TS211399 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | Spleen |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c (B cell); BALB/c (myeloma) |
| Applications： | The antibody precipitates the p62 c-myc protein, but does not react with mouse or chicken c-myc. |
| Derivation： | Mice were immunized with a synthetic peptide derived using the sequence of the human c-myc gene product. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human myc (c-myc) protein |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human myc (c-myc) protein |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal:xa0Every 2 to 3 days |
| Isotype： | IgG1 |
| Name of Depositor： | J Bishop, GI Evan |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Evan GI, et al. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616, 1985. PubMed: 3915782 Van Ewijk WV, et al. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments. Proc. Natl. Acad. Sci. USA 94: 3903-3908, 1997. PubMed: 9108077 Cheng M, et al. ERK3 is a constitutively nuclear protein kinase. J. Biol. Chem. 271: 8951-8958, 1996. PubMed: 8621539 |
