你好,请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
联系电话: call_new 0574-87917803
MRC-9
MRC-9
规格:
货期:
编号:TS211422
品牌:Testobio
产品名称: MRC-9
商品货号: TS211422
Organism: Homo sapiens, human
Tissue: lung
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: normal
Age: 15 weeks gestation
Gender: female
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: normal female; diploid; stable
Clinical Data:
15 weeks gestation
Caucasian
female
Tumorigenic: No
Effects:
No, in immunosuppressed mice
Yes, in semisolid medium
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 12
D5S818: 12,13
D7S820: 10,11
THO1: 6,8
TPOX: 11
vWA: 16,17
Population Doubling Capacity: 44 PDL
Name of Depositor: JP Jacobs
Deposited As: Homo sapiens
Year of Origin: May, 1974
References:

Jacobs JP, et al. Characteristics of a serially propagated human diploid cell designated MRC-9. J. Biol. Stand. 7: 113-122, 1979. PubMed: 113411

首页 > 产品中心 > 微生物培养 > 菌株 > null > MRC-9

MRC-9

  • 货号: TS211422
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: MRC-9
商品货号: TS211422
Organism: Homo sapiens, human
Tissue: lung
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: normal
Age: 15 weeks gestation
Gender: female
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: normal female; diploid; stable
Clinical Data:
15 weeks gestation
Caucasian
female
Tumorigenic: No
Effects:
No, in immunosuppressed mice
Yes, in semisolid medium
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Temperature: 37°C
STR Profile:
Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 12
D5S818: 12,13
D7S820: 10,11
THO1: 6,8
TPOX: 11
vWA: 16,17
Population Doubling Capacity: 44 PDL
Name of Depositor: JP Jacobs
Deposited As: Homo sapiens
Year of Origin: May, 1974
References:

Jacobs JP, et al. Characteristics of a serially propagated human diploid cell designated MRC-9. J. Biol. Stand. 7: 113-122, 1979. PubMed: 113411

合作单位: