| 产品名称： | MOPC-31C |
|---|---|
| 商品货号： | TS211435 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/cJ |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin |
| Cellular Products： | immunoglobulin |
| Virus Resistance： | poliovirus 3 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Leibovitzs L-15 medium with 2mM L-glutamine, 80%; fetal bovine serum, 20% (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Cultures can be maintained by addition of fresh medium, or by collecting cells as a pellet after centrifugation. The packed cells are then resuspended in fresh medium. The initial inoculum should not be less than 2 to 3 x 105 viable cells/mL. A maximum of 1 to 2 x 106 cells/mL are obtainable. Do not aspirate suspension to break up clumped cells. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days |
| Cryopreservation： | 90% FBS,10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C Atmosphere: Air 100% |
| Isotype： | IgG1 |
| Name of Depositor： | RJ Klebe |
| Deposited As： | Mus musculus |
| References： | Yosida TH, et al. Chromosomal alteration and development of tumors. XIX. Chromosome constitution of tumor cells in 16 plasma cell neoplasms of BALB/c mice. J. Natl. Cancer Inst. 41: 1083-1097, 1968. PubMed: 4176942 Potter M, Robertson CL. Development of plasma-cell neoplasms in BALB/c mice after intraperitoneal injection of paraffin-oil adjuvant, heart-killed Staphylococcus mixtures. J. Natl. Cancer Inst. 25: 847-861, 1960. PubMed: 13737512 Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | MOPC-31C |
|---|---|
| 商品货号： | TS211435 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/cJ |
| Storage Conditions： | liquid nitrogen vapor phase |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin |
| Cellular Products： | immunoglobulin |
| Virus Resistance： | poliovirus 3 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Leibovitzs L-15 medium with 2mM L-glutamine, 80%; fetal bovine serum, 20% (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Cultures can be maintained by addition of fresh medium, or by collecting cells as a pellet after centrifugation. The packed cells are then resuspended in fresh medium. The initial inoculum should not be less than 2 to 3 x 105 viable cells/mL. A maximum of 1 to 2 x 106 cells/mL are obtainable. Do not aspirate suspension to break up clumped cells. Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days |
| Cryopreservation： | 90% FBS,10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions： | Temperature: 37°C Atmosphere: Air 100% |
| Isotype： | IgG1 |
| Name of Depositor： | RJ Klebe |
| Deposited As： | Mus musculus |
| References： | Yosida TH, et al. Chromosomal alteration and development of tumors. XIX. Chromosome constitution of tumor cells in 16 plasma cell neoplasms of BALB/c mice. J. Natl. Cancer Inst. 41: 1083-1097, 1968. PubMed: 4176942 Potter M, Robertson CL. Development of plasma-cell neoplasms in BALB/c mice after intraperitoneal injection of paraffin-oil adjuvant, heart-killed Staphylococcus mixtures. J. Natl. Cancer Inst. 25: 847-861, 1960. PubMed: 13737512 Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
