| 产品名称： | MPC 11 OUAr |
|---|---|
| 商品货号： | TS211436 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin; monoclonal antibody,H-2d |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | M Cohn |
| Deposited As： | Mus musculus |
| References： | Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836 Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146 Gefter ML, et al. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet. 3: 231-236, 1977. PubMed: 605383 Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516 |
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| 产品名称： | MPC 11 OUAr |
|---|---|
| 商品货号： | TS211436 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | BALB/c |
| Applications： | This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). |
| Antigen Expression： | H-2d |
| Genes Expressed： | immunoglobulin; monoclonal antibody,H-2d |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | This is a ouabain resistant derivative of a cell line (MPC 11) established from the Merwin plasma cell tumor-11 (induced in a BALB/c mouse). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | M Cohn |
| Deposited As： | Mus musculus |
| References： | Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836 Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146 Gefter ML, et al. A simple method for polyethylene glycol-promoted hybridization of mouse myeloma cells. Somatic Cell Genet. 3: 231-236, 1977. PubMed: 605383 Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516 |
