Mm5MT

规格:

货期:

编号:TS211442

品牌:Testobio

产品名称：	Mm5MT
商品货号：	TS211442
Organism：	Mus musculus, mouse
Tissue：	mammary gland
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender：	female
Strain：	C3H/Crgl
Applications：	Tested and found negative for ectromelia virus (mousepox).
Storage Conditions：	liquid nitrogen vapor phase
Clinical Data：	female
Genes Expressed：	mouse mammary tumor virus (MMTV) and MMTV antigens
Cellular Products：	mouse mammary tumor virus (MMTV) and MMTV antigens
Comments：	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	RB Owens
Deposited As：	Mus musculus
References：	Owens RB, Hackett AJ. Tissue culture studies of mouse mammary tumor cells and associated viruses. J. Natl. Cancer Inst. 49: 1321-1332, 1972. PubMed: 4346536 Arthur LO, et al. Immunological characterization of the low-molecular-weight DNA binding protein of mouse mammary tumor virus. Int. J. Cancer 22: 433-440, 1978. PubMed: 81189 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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Mm5MT

货号: TS211442
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产品名称：	Mm5MT
商品货号：	TS211442
Organism：	Mus musculus, mouse
Tissue：	mammary gland
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Gender：	female
Strain：	C3H/Crgl
Applications：	Tested and found negative for ectromelia virus (mousepox).
Storage Conditions：	liquid nitrogen vapor phase
Clinical Data：	female
Genes Expressed：	mouse mammary tumor virus (MMTV) and MMTV antigens
Cellular Products：	mouse mammary tumor virus (MMTV) and MMTV antigens
Comments：	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney,xa05th edition, published by Wiley-Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	RB Owens
Deposited As：	Mus musculus
References：	Owens RB, Hackett AJ. Tissue culture studies of mouse mammary tumor cells and associated viruses. J. Natl. Cancer Inst. 49: 1321-1332, 1972. PubMed: 4346536 Arthur LO, et al. Immunological characterization of the low-molecular-weight DNA binding protein of mouse mammary tumor virus. Int. J. Cancer 22: 433-440, 1978. PubMed: 81189 Hay RJ, Caputo JL, Macy, ML, Eds. (1992) ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988 Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.