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MDA-MB-468
MDA-MB-468
规格:
货期:
编号:TS211478
品牌:Testobio
产品名称： MDA-MB-468
商品货号： TS211478
Organism： Homo sapiens, human
Tissue：
mammary gland/breast; derived from metastatic site: pleural effusion
Product Format： frozen
Morphology： epithelial
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： adenocarcinoma
Age： 51 years
Gender： female
Ethnicity： Black
Applications：
This cell line is a suitable transfection host.
Storage Conditions： liquid nitrogen vapor phase
Karyotype： modal number = 64; range = 60 to 67.
The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype.
Images：
Derivation：
The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast.
Clinical Data：
51 years
Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype.
Black
female
Antigen Expression：

Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient)
Receptor Expression：

epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha)
Tumorigenic： Yes
Effects：
Yes, in nude mice inoculated subcutaneously with 10(7) cells.
(Tumors developed within 21 days at 100% frequency (5/5).)
Comments：
Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. EGF receptor is present at 1 X 106 per cell.
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing： Volumes are given for a 75 cm2xa0flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
    Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
    Medium Renewal: 2 to 3 times per week
    Cryopreservation：
    Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions：
    Atmosphere: air, 100%
    Temperature: 37°C
    STR Profile：
    Amelogenin: X
    CSF1PO: 12
    D13S317: 12
    D16S539: 9
    D5S818: 12
    D7S820: 8
    THO1: 7
    TPOX: 8,9
    vWA: 18
    Isoenzymes：
    AK-1, 1
    ES-D, 1
    G6PD, A
    GLO-I, 1-2
    Me-2, 1-2
    PGM1, 1
    PGM3, 2
    Name of Depositor： R Cailleau
    Deposited As： Homo sapiens
    Year of Origin： 1977
    References：

    Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

    Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

    Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262

    Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

    Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845

    Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

    Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591

    Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

    Zamora-Leon SP, et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847

    Tumors developed within 21 days at 100% frequency (5/5).

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    MDA-MB-468

    • 货号: TS211478
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称： MDA-MB-468
    商品货号： TS211478
    Organism： Homo sapiens, human
    Tissue：
    mammary gland/breast; derived from metastatic site: pleural effusion
    Product Format： frozen
    Morphology： epithelial
    Culture Properties： adherent
    Biosafety Level： 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
    Disease： adenocarcinoma
    Age： 51 years
    Gender： female
    Ethnicity： Black
    Applications：
    This cell line is a suitable transfection host.
    Storage Conditions： liquid nitrogen vapor phase
    Karyotype： modal number = 64; range = 60 to 67.
    The cell line is aneuploid human, presumably female (X, abnormal X) with most chromosome counts in the hypotriploid range.; Normal chromosomes X, N2, N3, N7, N8, N10, and N22 are clearly under-represented due to their involvement in the formation of the many marker (19) chromosomes present in this cell line.; A normal chromosome N1 (or two) is identified in each karyotype, but, in addition, regions of chromosome N1 are also present in five different marker chromosomes.; Variation is evident in the normal and marker chromosome copy number from karyotype to karyotype.
    Images：
    Derivation：
    The MDA-MB-468 cell line was isolated in 1977 by R. Cailleau, et al., from a pleural effusion of a 51-year-old Black female patient with metastatic adenocarcinoma of the breast.
    Clinical Data：
    51 years
    Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype.
    Black
    female
    Antigen Expression：

    Blood Type AB; HLA Aw23, Aw30, B27, Bw35, Cw2, Cw4 (patient)
    Receptor Expression：

    epidermal growth factor (EGF); transforming growth factor alpha (TGF alpha)
    Tumorigenic： Yes
    Effects：
    Yes, in nude mice inoculated subcutaneously with 10(7) cells.
    (Tumors developed within 21 days at 100% frequency (5/5).)
    Comments：
    Although the tissue donor was heterozygous for the G6PD alleles, the cell line consistently showed only the G6PD A phenotype. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. EGF receptor is present at 1 X 106 per cell.
    Complete Growth Medium： The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

    (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


    Subculturing： Volumes are given for a 75 cm2xa0flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
    4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37°C.
      Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
      Medium Renewal: 2 to 3 times per week
      Cryopreservation：
      Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage temperature: liquid nitrogen vapor phase
      Culture Conditions：
      Atmosphere: air, 100%
      Temperature: 37°C
      STR Profile：
      Amelogenin: X
      CSF1PO: 12
      D13S317: 12
      D16S539: 9
      D5S818: 12
      D7S820: 8
      THO1: 7
      TPOX: 8,9
      vWA: 18
      Isoenzymes：
      AK-1, 1
      ES-D, 1
      G6PD, A
      GLO-I, 1-2
      Me-2, 1-2
      PGM1, 1
      PGM3, 2
      Name of Depositor： R Cailleau
      Deposited As： Homo sapiens
      Year of Origin： 1977
      References：

      Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

      Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

      Pathak S, et al. A human breast adenocarcinoma with chromosome and isoenzyme markers similar to those of the HeLa line. J. Natl. Cancer Inst. 62: 263-271, 1979. PubMed: 283262

      Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

      Nigro JM, et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 2531845

      Bates SE, et al. Expression of the transforming growth factor-alpha/epidermal growth factor receptor pathway in normal human breast epithelial cells. Endocrinology 126: 596-607, 1990. PubMed: 2294006

      Avila MA, et al. Quercetin mediates the down-regulation of mutant p53 in the human breast cancer cell line MDA-MB468. Cancer Res. 54: 2424-2428, 1994. PubMed: 8162591

      Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764

      Zamora-Leon SP, et al. Expression of the fructose transporter GLUT5 in human breast cancer. Proc. Natl. Acad. Sci. USA 93: 1847-1852, 1996. PubMed: 8700847

      Tumors developed within 21 days at 100% frequency (5/5).

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