| 产品名称： | MCF-10-2A |
|---|---|
| 商品货号： | TS211499 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrocystic disease |
| Age： | 36 years |
| Gender： | female |
| Applications： | This is a suitable transfection host. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype： | modal number = 40 to 43; XX; five marker chromosomes; double minutes |
| Derivation： | MCA-10-2A was derived from adherent cells in the population. The line was produced by long term culture in serum free medium with low Ca++ concentration. This line was established from the same tumor material as MCF 10A (ATCC CRL-10317) and MCF 10F (ATCC CRL-10318). |
| Clinical Data： | female |
| Receptor Expression： | epidermal growth factor (EGF); insulin; glucocorticoid |
| Genes Expressed： | cytokeratin 19 |
| Cellular Products： | cytokeratin 19 |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments： | The MCF-10-2A cell line is a non-tumorigenic epithelial cell line that does not undergo senescence after exposure to high calcium levels. The cells are positive for epithelial cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and infrequently form domes in confluent cultures. |
| Complete Growth Medium： | These cells are grown in a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hamx92s F12 medium (DMEM: F12 Medium; ATCC 30-2006). Supplemented with:
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 9 D16S539: 11,12 D5S818: 10,13 D7S820: 10,11 THO1: 8 TPOX: 9,11 vWA: 15,17 |
| Name of Depositor： | Michigan Cancer Foundation |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991 Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993 Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,238,840 dated Aug 24 1993 Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007 Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513 Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506 |
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| 产品名称： | MCF-10-2A |
|---|---|
| 商品货号： | TS211499 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | Epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrocystic disease |
| Age： | 36 years |
| Gender： | female |
| Applications： | This is a suitable transfection host. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype： | modal number = 40 to 43; XX; five marker chromosomes; double minutes |
| Derivation： | MCA-10-2A was derived from adherent cells in the population. The line was produced by long term culture in serum free medium with low Ca++ concentration. This line was established from the same tumor material as MCF 10A (ATCC CRL-10317) and MCF 10F (ATCC CRL-10318). |
| Clinical Data： | female |
| Receptor Expression： | epidermal growth factor (EGF); insulin; glucocorticoid |
| Genes Expressed： | cytokeratin 19 |
| Cellular Products： | cytokeratin 19 |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments： | The MCF-10-2A cell line is a non-tumorigenic epithelial cell line that does not undergo senescence after exposure to high calcium levels. The cells are positive for epithelial cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and infrequently form domes in confluent cultures. |
| Complete Growth Medium： | These cells are grown in a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hamx92s F12 medium (DMEM: F12 Medium; ATCC 30-2006). Supplemented with:
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 9 D16S539: 11,12 D5S818: 10,13 D7S820: 10,11 THO1: 8 TPOX: 9,11 vWA: 15,17 |
| Name of Depositor： | Michigan Cancer Foundation |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991 Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993 Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,238,840 dated Aug 24 1993 Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007 Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513 Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506 |
