| 产品名称： | MC116 |
|---|---|
| 商品货号： | TS211511 |
| Organism： | Homo sapiens, human |
| Tissue： | ascites |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | undifferentiated lymphoma |
| Gender： | male |
| Karyotype： | 45,XO/46,XY, dup1(q21-32), t(8;14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23) |
| Clinical Data： | male |
| Antigen Expression： | HLA A1, A3, B8, Bw35 |
| Genes Expressed： | immunoglobulin (surface and secreted) |
| Cellular Products： | immunoglobulin (surface and secreted) |
| Comments： | The cells are EBNA negative, negative for Fc receptors and negative for complement receptors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 105 viable cells/mL and maintain at or below 3 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Isotype： | IgM; lambda light chain |
| Population Doubling Time： | 26 to 28 hrs |
| Name of Depositor： | IT Magrath |
| Deposited As： | Homo sapiens |
| References： | Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722 |
| Cross References： | Nucleotide (GenBank) : U07991 Human lymphoma immunoglobulin lambda light chain mRNA, partial cds. Nucleotide (GenBank) : U07988 Human lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds. |
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| 产品名称： | MC116 |
|---|---|
| 商品货号： | TS211511 |
| Organism： | Homo sapiens, human |
| Tissue： | ascites |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | undifferentiated lymphoma |
| Gender： | male |
| Karyotype： | 45,XO/46,XY, dup1(q21-32), t(8;14)(8pter --> 8q23 :: 14q32 --> 14qter ; 14pter --> 14q32 :: 8q23 --> 8qter), del10(a23) |
| Clinical Data： | male |
| Antigen Expression： | HLA A1, A3, B8, Bw35 |
| Genes Expressed： | immunoglobulin (surface and secreted) |
| Cellular Products： | immunoglobulin (surface and secreted) |
| Comments： | The cells are EBNA negative, negative for Fc receptors and negative for complement receptors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing： | Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 3 X 105 viable cells/mL and maintain at or below 3 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Isotype： | IgM; lambda light chain |
| Population Doubling Time： | 26 to 28 hrs |
| Name of Depositor： | IT Magrath |
| Deposited As： | Homo sapiens |
| References： | Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitts and non-Burkitts type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J. Natl. Cancer Inst. 64: 465-476, 1980. PubMed: 6243721 Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722 |
| Cross References： | Nucleotide (GenBank) : U07991 Human lymphoma immunoglobulin lambda light chain mRNA, partial cds. Nucleotide (GenBank) : U07988 Human lymphoma immunoglobulin IgM heavy chain Fd fragment mRNA, partial cds. |
