MCB3901 [AV12-664]

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货期:

编号:TS211514

品牌:Testobio

产品名称：	MCB3901 AV12-664
商品货号：	TS211514
Organism：	Mesocricetus auratus, hamster, Syrian golden
Cell Type：	adenovirus 12 induced tumor
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 xa0Cells contain Adenovirus Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications：	This cell line can be used as a transfection host for exogenous gene expression using the BK virus enhancer - adenovirus late promoter system.
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype：	modal chromosome number is approximately 46
Genes Expressed：	early region proteins of adenovirus 12
Cellular Products：	early region proteins of adenovirus 12
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:8 to 1:20 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Culture Conditions：	Temperature: 37°C
Population Doubling Time：	14 to 16 hrs
Name of Depositor：	Eli Lilly & Co.
Deposited As：	Mesocricetus auratus
U.S. Patent Number：	4,975,369
References：	Beavers LS, et al. Recombinant and chimeric KS1/4 antibodies directed against a human adenocarcinoma antigen. US Patent 4,975,369 dated Dec 4 1990 Berg DT, et al. E1a-responsive mammalian host/vector system for the stable high-level expression of secreted proteins. Nucleic Acids Res. 20: 5485-5486, 1992. PubMed: 1437573 Grinnell BW, et al. Gamma-carboxylated isoforms of recombinant human protein S with different biologic properties. Blood 76: 2546-2554, 1990. PubMed: 2148275 Burck PJ, et al. Characterization of a modified human tissue plasminogen activator comprising a kringle-2 and a protease domain. J. Biol. Chem. 265: 5170-5177, 1990. PubMed: 2108167 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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MCB3901 [AV12-664]

货号: TS211514
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产品名称：	MCB3901 AV12-664
商品货号：	TS211514
Organism：	Mesocricetus auratus, hamster, Syrian golden
Cell Type：	adenovirus 12 induced tumor
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 xa0Cells contain Adenovirus Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications：	This cell line can be used as a transfection host for exogenous gene expression using the BK virus enhancer - adenovirus late promoter system.
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype：	modal chromosome number is approximately 46
Genes Expressed：	early region proteins of adenovirus 12
Cellular Products：	early region proteins of adenovirus 12
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:8 to 1:20 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Culture Conditions：	Temperature: 37°C
Population Doubling Time：	14 to 16 hrs
Name of Depositor：	Eli Lilly & Co.
Deposited As：	Mesocricetus auratus
U.S. Patent Number：	4,975,369
References：	Beavers LS, et al. Recombinant and chimeric KS1/4 antibodies directed against a human adenocarcinoma antigen. US Patent 4,975,369 dated Dec 4 1990 Berg DT, et al. E1a-responsive mammalian host/vector system for the stable high-level expression of secreted proteins. Nucleic Acids Res. 20: 5485-5486, 1992. PubMed: 1437573 Grinnell BW, et al. Gamma-carboxylated isoforms of recombinant human protein S with different biologic properties. Blood 76: 2546-2554, 1990. PubMed: 2148275 Burck PJ, et al. Characterization of a modified human tissue plasminogen activator comprising a kringle-2 and a protease domain. J. Biol. Chem. 265: 5170-5177, 1990. PubMed: 2108167 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.