Malme-3

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货期:

编号:TS211541

品牌:Testobio

产品名称：	Malme-3
商品货号：	TS211541
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	43 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived. Thus, the two lines provide normal and melanoma tumor counterparts for comparative in vitro studies.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	This is a normal diploid human cell line with 46, XY karyotype and the modal chromosome number of 46 occurring in 78% of cells. No marker chromosomes were detected. Both X and Y chromosomes were single-copied and normal in morphology.
Derivation：	This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived.
Clinical Data：	43 years adult Caucasian male
Antigen Expression：	HLA A2, Aw30, B13, B40(+/-), DRw7
Genes Expressed：	HLA A2, Aw30, B13, B40(+/-), DRw7
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Interval: Subculture every 6 to 8 days Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,13 D16S539: 9,12 D5S818: 11,13 D7S820: 9,12 THO1: 7,8 TPOX: 8,9 vWA: 15,16
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1
Name of Depositor：	J Fogh
Deposited As：	Homo sapiens
References：	Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.

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Malme-3

货号: TS211541
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产品名称：	Malme-3
商品货号：	TS211541
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	43 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived. Thus, the two lines provide normal and melanoma tumor counterparts for comparative in vitro studies.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	This is a normal diploid human cell line with 46, XY karyotype and the modal chromosome number of 46 occurring in 78% of cells. No marker chromosomes were detected. Both X and Y chromosomes were single-copied and normal in morphology.
Derivation：	This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived.
Clinical Data：	43 years adult Caucasian male
Antigen Expression：	HLA A2, Aw30, B13, B40(+/-), DRw7
Genes Expressed：	HLA A2, Aw30, B13, B40(+/-), DRw7
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Interval: Subculture every 6 to 8 days Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,13 D16S539: 9,12 D5S818: 11,13 D7S820: 9,12 THO1: 7,8 TPOX: 8,9 vWA: 15,16
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1
Name of Depositor：	J Fogh
Deposited As：	Homo sapiens
References：	Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.