MAR 18.5
MAR 18.5
规格:
货期:
编号:TS211545
品牌:Testobio
| 产品名称： | MAR 18.5 |
|---|---|
| 商品货号： | TS211545 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rat kappa light chain (RI-1a and RI-1b allotypes) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rat kappa light chain (RI-1a and RI-1b allotypes) |
| Comments： | Animals were immunized with soluble rat immunoglobulin. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | LL Lanier |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lanier LL, et al. Monoclonal antibodies against rat immunoglobulin kappa chains. Hybridoma 1: 125-131, 1982. PubMed: 6821397 |
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MAR 18.5
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | MAR 18.5 |
|---|---|
| 商品货号： | TS211545 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against rat kappa light chain (RI-1a and RI-1b allotypes) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against rat kappa light chain (RI-1a and RI-1b allotypes) |
| Comments： | Animals were immunized with soluble rat immunoglobulin. Spleen cells were fused with P3X63Ag8 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | LL Lanier |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lanier LL, et al. Monoclonal antibodies against rat immunoglobulin kappa chains. Hybridoma 1: 125-131, 1982. PubMed: 6821397 |
