| 产品名称： | MA-104 Clone 1 |
|---|---|
| 商品货号： | TS211567 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. |
| Virus Susceptibility： | Simian rotavirus
|
| Comments： | This item is not available for international distribution. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:10 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Population Doubling Time： | 27 hours |
| Name of Depositor： | MM Vincent |
| Year of Origin： | 2001 |
| References： | Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530 MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. Chromosome and isoenzyme analysis of the cells distributed by some sources showed that the species of origin was African green monkey (PubMed: 4043530). In May of 1994, M.M. Vincent deposited ampules of MA-104 at passage 3 at the ATCC. At passage 16 and higher, presence of both African green monkey and Rhesus monkey cells were detected by isoenzyme analysis. An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. (ATCC CRL-2378). This line is available as MA-104 clone 1 (CRL-2378.1). |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | MA-104 Clone 1 |
|---|---|
| 商品货号： | TS211567 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. |
| Virus Susceptibility： | Simian rotavirus
|
| Comments： | This item is not available for international distribution. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:10 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Population Doubling Time： | 27 hours |
| Name of Depositor： | MM Vincent |
| Year of Origin： | 2001 |
| References： | Whitaker AM, Hayward CJ. The characterization of three monkey kidney cell lines. Dev. Biol. Stand. 60: 125-131, 1985. PubMed: 4043530 MA-104 was developed by initial explant culture and subsequent passage by enzymatic dissociation at Microbiological Associates from embryonic Rhesus monkey kidney tissue. Chromosome and isoenzyme analysis of the cells distributed by some sources showed that the species of origin was African green monkey (PubMed: 4043530). In May of 1994, M.M. Vincent deposited ampules of MA-104 at passage 3 at the ATCC. At passage 16 and higher, presence of both African green monkey and Rhesus monkey cells were detected by isoenzyme analysis. An African green monkey pure cell population was isolated at the ATCC by cloning MA-104. (ATCC CRL-2378). This line is available as MA-104 clone 1 (CRL-2378.1). |
