LYK-9
LYK-9
规格:
货期:
编号:TS211591
品牌:Testobio
| 产品名称： | LYK-9 |
|---|---|
| 商品货号： | TS211591 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: S344NCR (B cell); BALB/c (myeloma) |
| Applications： | Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10. |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10. |
| Comments： | Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with:
0.05 mM 2-mercaptoethanol
1% Origen Hybridoma Cloning Factor
10% fetal bovine serum
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a |
| Name of Depositor： | KS Hathcock, Y Yamashita |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Yamashita Y, et al. Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314 |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
LYK-9
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | LYK-9 |
|---|---|
| 商品货号： | TS211591 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: S344NCR (B cell); BALB/c (myeloma) |
| Applications： | Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10. |
| Cellular Products： | immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10. |
| Comments： | Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with:
0.05 mM 2-mercaptoethanol
1% Origen Hybridoma Cloning Factor
10% fetal bovine serum
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a |
| Name of Depositor： | KS Hathcock, Y Yamashita |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Yamashita Y, et al. Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314 |
