LS 102.9
LS 102.9
规格:
货期:
编号:TS211617
品牌:Testobio
| 产品名称： | LS 102.9 |
|---|---|
| 商品货号： | TS211617 |
| Organism： | Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). |
| Comments： | The LS 102.9 bears surface I-A d,s and I-E d molecules and can present antigen to appropriate I region restricted T cell hybridomas. The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Modified Dulbeccos medium (see below) with 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. DME w/HEPES (10mM) 100 mL L-Glutamine (100x) 1 mL Solution I 1 mL Non-essential amino acids (100x) 1 mL NCTC 135 10 mL Fetal Bovine Serum 12 mL Solution I (100x) 1. 1320 mg oxalacetic acid (100 mM, MW 132). 2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg). Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to 100 mL with distilled water. Stir at 37°C until solution dissolves. Filter, aliquot and store frozen. |
| Subculturing： | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml Maintain pH below 7.2. |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | P Marrack, J Kappler |
| Deposited As： | mouse (B cell); mouse (lymphoma) |
| References： | Kappler J, et al. Antigen presentation by Ia+ B cell hybridomas to H-2 restricted T cell hybridomas. Proc. Natl. Acad. Sci. USA 79: 3604-3607, 1982. PubMed: 6980413 |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | LS 102.9 |
|---|---|
| 商品货号： | TS211617 |
| Organism： | Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). |
| Comments： | The LS 102.9 bears surface I-A d,s and I-E d molecules and can present antigen to appropriate I region restricted T cell hybridomas. The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.S(7R) mice (I-As, I-Es). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: Modified Dulbeccos medium (see below) with 50 uM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. DME w/HEPES (10mM) 100 mL L-Glutamine (100x) 1 mL Solution I 1 mL Non-essential amino acids (100x) 1 mL NCTC 135 10 mL Fetal Bovine Serum 12 mL Solution I (100x) 1. 1320 mg oxalacetic acid (100 mM, MW 132). 2. 80 mg crystalline bovine insulin (20 units/mL, 25 units/mg). Add 1 and 2, stir at 37°C. Add Na pyruvate 550 mg (50 mM, FW 110). Bring up to 100 mL with distilled water. Stir at 37°C until solution dissolves. Filter, aliquot and store frozen. |
| Subculturing： | Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml Maintain pH below 7.2. |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | P Marrack, J Kappler |
| Deposited As： | mouse (B cell); mouse (lymphoma) |
| References： | Kappler J, et al. Antigen presentation by Ia+ B cell hybridomas to H-2 restricted T cell hybridomas. Proc. Natl. Acad. Sci. USA 79: 3604-3607, 1982. PubMed: 6980413 |
