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LL 97A (AlMy)
LL 97A (AlMy)
规格:
货期:
编号:TS211634
品牌:Testobio
产品名称: LL 97A (AlMy)
商品货号: TS211634
Organism: Homo sapiens, human
Tissue: lung
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: Idiopathic pulmonary fibrosis
Age: 48 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: diploid; relatively unstable
Clinical Data:
48 years
Caucasian
male
Complete Growth Medium: Hams F12K medium, 85%; fetal bovine serum, 15%
Subculturing:

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0

  1. Remove and discard culture medium.xa0
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 11,13
D5S818: 13
D7S820: 9,11
THO1: 6,9.3
TPOX: 10,11
vWA: 14,17
Isoenzymes:
G6PD, B
Name of Depositor: RG Crystal
Deposited As: Homo sapiens
首页 > 产品中心 > 微生物培养 > 菌株 > null > LL 97A (AlMy)

LL 97A (AlMy)

  • 货号: TS211634
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: LL 97A (AlMy)
商品货号: TS211634
Organism: Homo sapiens, human
Tissue: lung
Cell Type: fibroblast
Product Format: frozen
Morphology: fibroblast
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: Idiopathic pulmonary fibrosis
Age: 48 years
Gender: male
Ethnicity: Caucasian
Storage Conditions: liquid nitrogen vapor phase
Karyotype: diploid; relatively unstable
Clinical Data:
48 years
Caucasian
male
Complete Growth Medium: Hams F12K medium, 85%; fetal bovine serum, 15%
Subculturing:

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0

  1. Remove and discard culture medium.xa0
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 11,13
D5S818: 13
D7S820: 9,11
THO1: 6,9.3
TPOX: 10,11
vWA: 14,17
Isoenzymes:
G6PD, B
Name of Depositor: RG Crystal
Deposited As: Homo sapiens
合作单位: