| 产品名称： | LLC-MK2 Original |
|---|---|
| 商品货号： | TS211636 |
| Organism： | Macaca mulatta, monkey, rhesus |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | adult |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Genes Expressed： | plasminogen activator |
| Cellular Products： | plasminogen activator |
| Virus Susceptibility： | Bovine viral diarrhea virus 1
,
Bovine viral diarrhea virus 1 Human poliovirus 1 Human Coxsackievirus B4 |
| Comments： | The serum used in the medium should be pre-tested for good quality.
Some lots of horse serum do support satisfactory growth.
Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth. |
| Complete Growth Medium： | Medium 199 (with 1.68 g/L bicarbonate), 99%; horse serum, 1%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | RN Hull |
| Deposited As： | Macaca mulatta |
| References： | American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956. Hull RN, et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 14449901 Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902 |
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| 产品名称： | LLC-MK2 Original |
|---|---|
| 商品货号： | TS211636 |
| Organism： | Macaca mulatta, monkey, rhesus |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Normal |
| Age： | adult |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： | |
| Genes Expressed： | plasminogen activator |
| Cellular Products： | plasminogen activator |
| Virus Susceptibility： | Bovine viral diarrhea virus 1
,
Bovine viral diarrhea virus 1 Human poliovirus 1 Human Coxsackievirus B4 |
| Comments： | The serum used in the medium should be pre-tested for good quality.
Some lots of horse serum do support satisfactory growth.
Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth. |
| Complete Growth Medium： | Medium 199 (with 1.68 g/L bicarbonate), 99%; horse serum, 1%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: Every 2 to 3 days
|
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | RN Hull |
| Deposited As： | Macaca mulatta |
| References： | American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956. Hull RN, et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 14449901 Hull RN, Tritch OJ. Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed: 14449902 |
