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LLC-WRC 256
LLC-WRC 256
规格:
货期:
编号:TS211637
品牌:Testobio
产品名称: LLC-WRC 256
商品货号: TS211637
Organism: Rattus norvegicus, rat
Tissue: mammary gland
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: mammary gland; Walker carcinoma
Strain: Walker
Tumorigenic: Yes
Effects:
Yes, in Amsterdam rats
Virus Resistance:
poliovirus 1
Complete Growth Medium: Medium 199, 95%; horse serum, 5%
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor: RN Hull
Deposited As: Rattus norvegicus
References:

American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956.

Hull RN. Establishing long-term cultures of mammalian normal, solid tumor, and ascites tumor cells on glass. Science 117:223, 1953. Pubmed : 13038481

Hull RN, Cherry WR, Johnson IS. The adaptation and maintenance of mammalian cells to continuous growth in tissue culture. Anat Rec 124: 490–499,1956.

Earle WR. A study of the Walker rat mammary carcinoma 256, in vivo and in vitro. Am J Cancer 24:566-612, 1935.

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LLC-WRC 256

  • 货号: TS211637
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  • 品牌 : TESTOBIO
产品名称: LLC-WRC 256
商品货号: TS211637
Organism: Rattus norvegicus, rat
Tissue: mammary gland
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: mammary gland; Walker carcinoma
Strain: Walker
Tumorigenic: Yes
Effects:
Yes, in Amsterdam rats
Virus Resistance:
poliovirus 1
Complete Growth Medium: Medium 199, 95%; horse serum, 5%
Subculturing:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Name of Depositor: RN Hull
Deposited As: Rattus norvegicus
References:

American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956.

Hull RN. Establishing long-term cultures of mammalian normal, solid tumor, and ascites tumor cells on glass. Science 117:223, 1953. Pubmed : 13038481

Hull RN, Cherry WR, Johnson IS. The adaptation and maintenance of mammalian cells to continuous growth in tissue culture. Anat Rec 124: 490–499,1956.

Earle WR. A study of the Walker rat mammary carcinoma 256, in vivo and in vitro. Am J Cancer 24:566-612, 1935.

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