LL 24

规格:

货期:

编号:TS211647

品牌:Testobio

产品名称：	LL 24
商品货号：	TS211647
Organism：	Homo sapiens, human
Tissue：	lung
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	5 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen storage vapor
Karyotype：	normal human male; diploid; stable
Clinical Data：	male Caucasian 5 years
Complete Growth Medium：	Hams F12K medium, 85%; fetal bovine serum, 15% Other standard media (EMEM or McCoys 5a) may be used
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio ofxa01:2 to 1: 4 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994
STR Profile：	Amelogenin: X,Y CSF1PO: 9,12 D13S317: 8,11 D16S539: 9,12 D5S818: 10,11 D7S820: 11,12 THO1: 7 TPOX: 8 vWA: 16,18
Isoenzymes：	G6PD, B
Name of Depositor：	RG Crystal
Deposited As：	Homo sapiens
References：	Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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LL 24

货号: TS211647
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产品名称：	LL 24
商品货号：	TS211647
Organism：	Homo sapiens, human
Tissue：	lung
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	5 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen storage vapor
Karyotype：	normal human male; diploid; stable
Clinical Data：	male Caucasian 5 years
Complete Growth Medium：	Hams F12K medium, 85%; fetal bovine serum, 15% Other standard media (EMEM or McCoys 5a) may be used
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio ofxa01:2 to 1: 4 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994
STR Profile：	Amelogenin: X,Y CSF1PO: 9,12 D13S317: 8,11 D16S539: 9,12 D5S818: 10,11 D7S820: 11,12 THO1: 7 TPOX: 8 vWA: 16,18
Isoenzymes：	G6PD, B
Name of Depositor：	RG Crystal
Deposited As：	Homo sapiens
References：	Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.