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LC-540
LC-540
规格:
货期:
编号:TS211648
品牌:Testobio
产品名称: LC-540
商品货号: TS211648
Organism: Rattus norvegicus, rat
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age: adult
Gender: male
Strain: Fischer
Storage Conditions: liquid nitrogen vapor phase
Karyotype: modal number = 42; range = 40 to 43.
Approximately 21% of the cells examined were aneuploids. No other structural abnormalities were noted.
Clinical Data:
male
Tumorigenic: Yes
Effects:
Yes, in rats
Virus Susceptibility: Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
Virus Resistance:
poliovirus 1
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor: A Steinberger
Deposited As: Rattus sp.
Year of Origin: October, 1969
References:

Jacobs BB, Huseby RA. Transplantable Leydig cell tumors in Fischer rats: hormone responsibility and hormone production. J. Natl. Cancer Inst. 41: 1141-1153, 1968. PubMed: 5688092

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LC-540

  • 货号: TS211648
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: LC-540
商品货号: TS211648
Organism: Rattus norvegicus, rat
Product Format: frozen
Morphology: epithelial
Culture Properties: adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age: adult
Gender: male
Strain: Fischer
Storage Conditions: liquid nitrogen vapor phase
Karyotype: modal number = 42; range = 40 to 43.
Approximately 21% of the cells examined were aneuploids. No other structural abnormalities were noted.
Clinical Data:
male
Tumorigenic: Yes
Effects:
Yes, in rats
Virus Susceptibility: Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Human poliovirus 1
Virus Resistance:
poliovirus 1
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation:
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions:
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor: A Steinberger
Deposited As: Rattus sp.
Year of Origin: October, 1969
References:

Jacobs BB, Huseby RA. Transplantable Leydig cell tumors in Fischer rats: hormone responsibility and hormone production. J. Natl. Cancer Inst. 41: 1141-1153, 1968. PubMed: 5688092

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