| 产品名称： | LA-4 |
|---|---|
| 商品货号： | TS211659 |
| Organism： | Mus musculus, mouse |
| Tissue： | lung |
| Cell Type： | urethane induced |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenoma |
| Age： | 28 weeks |
| Strain： | A/He |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Unstable karyotype. Range = 38 to 256. Metacentric chromosomes are noted in about 12% of the cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | GD Stoner |
| Deposited As： | Mus musculus |
| References： | Stoner GD, et al. Clonal isolation of epithelial cells from mouse lung adenoma. Cancer Res. 35: 2177-2185, 1975. PubMed: 167947 Stoner GD, et al. Epithelial cells cultured from mouse lung adenoma: biosynthesis of lecithin and comparison of phospholipids with the lung. Exp. Mol. Pathol. 29: 102-114, 1978. PubMed: 581072 |
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| 产品名称： | LA-4 |
|---|---|
| 商品货号： | TS211659 |
| Organism： | Mus musculus, mouse |
| Tissue： | lung |
| Cell Type： | urethane induced |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenoma |
| Age： | 28 weeks |
| Strain： | A/He |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Unstable karyotype. Range = 38 to 256. Metacentric chromosomes are noted in about 12% of the cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Hams F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | GD Stoner |
| Deposited As： | Mus musculus |
| References： | Stoner GD, et al. Clonal isolation of epithelial cells from mouse lung adenoma. Cancer Res. 35: 2177-2185, 1975. PubMed: 167947 Stoner GD, et al. Epithelial cells cultured from mouse lung adenoma: biosynthesis of lecithin and comparison of phospholipids with the lung. Exp. Mol. Pathol. 29: 102-114, 1978. PubMed: 581072 |
