L2-RYC

规格:

货期:

编号:TS211670

品牌:Testobio

产品名称：	L2-RYC
商品货号：	TS211670
Organism：	Rattus norvegicus, rat
Tissue：	embryo
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Carcinoma
Age：	embryo; 10 days gestation
Strain：	Lewis
Applications：	The cell line expresses parietal yolk sac endoderm characteristics in that it synthesizes basement membrane components. Alpha fetoprotein synthesis could not be demonstrated.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	The majority of cells had a pseudodiploid chromosome complement; approximately 10% of cells were pseudotetraploid.
Derivation：	L2-RYC is a yolk sac carcinoma established in 1978 by Dr. Ulla Wewer from a yolk sac tumor induced by puncturing a Lewis rat embryo through the wall of the uterus at the 10th day of gestation. A yolk sac carcinoma developed approximately 71/2 months later. The L2 cell line in which only parietal carcinoma cells could be identified, was established from transplant number 14. In the early passages of the parental cell line, designated L1, both visceral and parietal yolk sac carcinomas were present.
Genes Expressed：	laminin; entactin; collagen IV; heparin sulfate proteoglycan; chondroitin sulfate proteoglycan
Cellular Products：	laminin; entactin; collagen IV; heparin sulfate proteoglycan; chondroitin sulfate proteoglycan
Tumorigenic：	Yes
Effects：	Yes, in Lewis rats
Comments：	L2-RYC is a yolk sac carcinoma established in 1978 by Dr. Ulla Wewer from a yolk sac tumor induced by puncturing a Lewis rat embryo through the wall of the uterus at the 10th day of gestation. A yolk sac carcinoma developed approximately 71/2 months later. In the early passages of the parental cell line, designated L1, both visceral and parietal yolk sac carcinomas were present. L1 cells were injected intraperitoneally into rats and serial transplantations were carried out. The L2 cell line in which only parietal carcinoma cells could be identified, was established from transplant number 14. The cell line expresses parietal yolk sac endoderm characteristics in that it synthesizes basement membrane components. In addition, a noncartilage chondroitin sulfate proteoglycan is synthesized. Alpha fetoprotein synthesis could not be demonstrated. The cells must be subcultured frequently and not be allowed to become dense.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:5 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	S Thorgeirsson
Deposited As：	Rattus sp.
Year of Origin：	1978
References：	Wewer UM, et al. Heparan sulfate proteoglycans made by different basement-membrane- producing tumors have immunological and structural similarities. Differentiation 30: 61-67, 1985. PubMed: 2936642 Wewer U. Characterization of a rat yolk sac carcinoma cell line. Dev. Biol. 93: 416-421, 1982. PubMed: 7141105 Wewer U, et al. Laminin, a noncollagenous component of epithelial basement membranes synthesized by a rat yolk sac tumor. Cancer Res. 41: 1518-1524, 1981. PubMed: 7011537 . . Acta Pathol. Scand. Immunol. Sect. A, Immunol. 92: 275-283, 1984. Oldberg A, et al. Isolation of a chondroitin sulfate proteoglycan from a rat yolk sac tumor and immunochemical demonstration of its cell surface localization. J. Biol. Chem. 256: 10847-10852, 1981. PubMed: 6793588 Lundstrom M, et al. Immunocytochemical and biochemical characterization of the Heymann nephritis antigenic complex in rat L2 yolk sac cells. Am. J. Pathol. 143: 1423-1435, 1993. PubMed: 8238258 Orlando RA, Farquhar MG. Identification of a cell line that expresses a cell surface and a soluble form of the gp330/receptor-associated protein (RAP) Heymann nephritis antigenic complex. Proc. Natl. Acad. Sci. USA 90: 4082-4086, 1993. PubMed: 8483924 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

首页 > 产品中心 > 微生物培养 > 菌株 > null > L2-RYC

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

L2-RYC

货号: TS211670
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	L2-RYC
商品货号：	TS211670
Organism：	Rattus norvegicus, rat
Tissue：	embryo
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Carcinoma
Age：	embryo; 10 days gestation
Strain：	Lewis
Applications：	The cell line expresses parietal yolk sac endoderm characteristics in that it synthesizes basement membrane components. Alpha fetoprotein synthesis could not be demonstrated.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	The majority of cells had a pseudodiploid chromosome complement; approximately 10% of cells were pseudotetraploid.
Derivation：	L2-RYC is a yolk sac carcinoma established in 1978 by Dr. Ulla Wewer from a yolk sac tumor induced by puncturing a Lewis rat embryo through the wall of the uterus at the 10th day of gestation. A yolk sac carcinoma developed approximately 71/2 months later. The L2 cell line in which only parietal carcinoma cells could be identified, was established from transplant number 14. In the early passages of the parental cell line, designated L1, both visceral and parietal yolk sac carcinomas were present.
Genes Expressed：	laminin; entactin; collagen IV; heparin sulfate proteoglycan; chondroitin sulfate proteoglycan
Cellular Products：	laminin; entactin; collagen IV; heparin sulfate proteoglycan; chondroitin sulfate proteoglycan
Tumorigenic：	Yes
Effects：	Yes, in Lewis rats
Comments：	L2-RYC is a yolk sac carcinoma established in 1978 by Dr. Ulla Wewer from a yolk sac tumor induced by puncturing a Lewis rat embryo through the wall of the uterus at the 10th day of gestation. A yolk sac carcinoma developed approximately 71/2 months later. In the early passages of the parental cell line, designated L1, both visceral and parietal yolk sac carcinomas were present. L1 cells were injected intraperitoneally into rats and serial transplantations were carried out. The L2 cell line in which only parietal carcinoma cells could be identified, was established from transplant number 14. The cell line expresses parietal yolk sac endoderm characteristics in that it synthesizes basement membrane components. In addition, a noncartilage chondroitin sulfate proteoglycan is synthesized. Alpha fetoprotein synthesis could not be demonstrated. The cells must be subcultured frequently and not be allowed to become dense.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:5 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	S Thorgeirsson
Deposited As：	Rattus sp.
Year of Origin：	1978
References：	Wewer UM, et al. Heparan sulfate proteoglycans made by different basement-membrane- producing tumors have immunological and structural similarities. Differentiation 30: 61-67, 1985. PubMed: 2936642 Wewer U. Characterization of a rat yolk sac carcinoma cell line. Dev. Biol. 93: 416-421, 1982. PubMed: 7141105 Wewer U, et al. Laminin, a noncollagenous component of epithelial basement membranes synthesized by a rat yolk sac tumor. Cancer Res. 41: 1518-1524, 1981. PubMed: 7011537 . . Acta Pathol. Scand. Immunol. Sect. A, Immunol. 92: 275-283, 1984. Oldberg A, et al. Isolation of a chondroitin sulfate proteoglycan from a rat yolk sac tumor and immunochemical demonstration of its cell surface localization. J. Biol. Chem. 256: 10847-10852, 1981. PubMed: 6793588 Lundstrom M, et al. Immunocytochemical and biochemical characterization of the Heymann nephritis antigenic complex in rat L2 yolk sac cells. Am. J. Pathol. 143: 1423-1435, 1993. PubMed: 8238258 Orlando RA, Farquhar MG. Identification of a cell line that expresses a cell surface and a soluble form of the gp330/receptor-associated protein (RAP) Heymann nephritis antigenic complex. Proc. Natl. Acad. Sci. USA 90: 4082-4086, 1993. PubMed: 8483924 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.