| 产品名称： | L5178-S (LY-S) |
|---|---|
| 商品货号： | TS211676 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | chemically induced |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | DBA/2 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from the X-ray resistant L5178-R line (ATCC CRL-1722). The parental cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse. |
| Tumorigenic： | Yes |
| Effects： | Yes, in DBA/2 mice (but only weakly). |
| Comments： | The cells are sensitive to X-rays, ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD). |
| Complete Growth Medium： | Fischers medium with 0.1 g/L sodium pyruvate and 1.125 sodium bicarbonate, 90%; horse serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1xa0x 104 cells/mL and maintain between 5xa0x 103 and 7xa0x 105 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium, 90%; DMSO, 10% |
| Name of Depositor： | JZ Beer |
| Deposited As： | Mus musculus |
| References： | Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899 Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332 Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | L5178-S (LY-S) |
|---|---|
| 商品货号： | TS211676 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | chemically induced |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | DBA/2 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from the X-ray resistant L5178-R line (ATCC CRL-1722). The parental cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse. |
| Tumorigenic： | Yes |
| Effects： | Yes, in DBA/2 mice (but only weakly). |
| Comments： | The cells are sensitive to X-rays, ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD). |
| Complete Growth Medium： | Fischers medium with 0.1 g/L sodium pyruvate and 1.125 sodium bicarbonate, 90%; horse serum, 10%
|
| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1xa0x 104 cells/mL and maintain between 5xa0x 103 and 7xa0x 105 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium, 90%; DMSO, 10% |
| Name of Depositor： | JZ Beer |
| Deposited As： | Mus musculus |
| References： | Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899 Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332 Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
