| 产品名称： | KL295 |
|---|---|
| 商品货号： | TS211704 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse Ab/beta protein (domain beta 1).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Cellular Products： | Immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Comments： | The antibody reacts with denatured beta chains of the b, d, p, q, j and bu haplotypes.
It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface. It does not react with molecules of the k, f, and s haplotypes. The reactive determinant is most likely involves residues 153 through 155. Depositor recommends using low IgG serum. ATCC uses ultra-low IgG fetal bovine serum (Invitrogen catalog # 16250). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Antigenic Determinant： | Ab/beta protein (domain beta 1), mouse |
| Name of Depositor： | JA Frelinger |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | KL295 |
|---|---|
| 商品货号： | TS211704 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse Ab/beta protein (domain beta 1).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Cellular Products： | Immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Comments： | The antibody reacts with denatured beta chains of the b, d, p, q, j and bu haplotypes.
It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface. It does not react with molecules of the k, f, and s haplotypes. The reactive determinant is most likely involves residues 153 through 155. Depositor recommends using low IgG serum. ATCC uses ultra-low IgG fetal bovine serum (Invitrogen catalog # 16250). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Antigenic Determinant： | Ab/beta protein (domain beta 1), mouse |
| Name of Depositor： | JA Frelinger |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
