KL304
KL304
规格:
货期:
编号:TS211706
品牌:Testobio
| 产品名称： | KL304 |
|---|---|
| 商品货号： | TS211706 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1). The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Comments： | Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes. It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface. It does not react with molecules of the b, d, p or q haplotypes. The reactive determinant most likely involves residues 153 through 155. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
|
| Subculturing： | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Subcultivation Ratio: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG2b |
| Name of Depositor： | JA Frelinger |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718 |
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周雪
13372109301
| 产品名称： | KL304 |
|---|---|
| 商品货号： | TS211706 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1). The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the beta chain of mouse Class II antigens |
| Comments： | Animals were immunized with synthetic peptide corresponding to residues 146 to 157 of the mouse As/beta protein (domain beta 1). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with denatured beta chains of the a, f, k, j, s and bw haplotypes. It does not detect the intact H-2b Class II molecule, and does not detect the beta chain on the cell surface. It does not react with molecules of the b, d, p or q haplotypes. The reactive determinant most likely involves residues 153 through 155. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 1% nonessential amino acids, 10 mM HEPES, 0.01 mM 2-mercaptoethanol and 10% fetal bovine serum
|
| Subculturing： | Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Subcultivation Ratio: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG2b |
| Name of Depositor： | JA Frelinger |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | LaPan KE, et al. Production and characterization of two new mouse monoclonal antibodies reactive with denatured mouse class II beta chains. Hybridoma 11: 217-223, 1992. PubMed: 1376718 |
