K-BALB (K-234)

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货期:

编号:TS211712

品牌:Testobio

产品名称：	K-BALB (K-234)
商品货号：	TS211712
Organism：	Mus musculus, mouse
Tissue：	embryo
Cell Type：	fibroblast, Kirsten murine sarcoma virus (Ki-MSV) transformed
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	14 to 17 days gestation embryo
Strain：	BALB/c
Storage Conditions：	liquid nitrogen vapor phase
Antigen Expression：	H-2d
Tumorigenic：	Yes
Effects：	Yes, in BALB/c mice
Comments：	They do not produce Ki-MSV.
Complete Growth Medium：	Dulbeccos modified Eagles medium, 90%; bovine calf serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Add 2.0 to 3.0 mL of Trypsin-EDTA solution (0.25% (w/v) Trypsin - 0.53mM EDTA) to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:8 Medium Renewal:xa0Twice a week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	S Aaronson
Deposited As：	Mus musculus
References：	Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712 Stephenson JR, Aaronson SA. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J. Exp. Med. 135: 503-515, 1972. PubMed: 4550769 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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K-BALB (K-234)

货号: TS211712
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产品名称：	K-BALB (K-234)
商品货号：	TS211712
Organism：	Mus musculus, mouse
Tissue：	embryo
Cell Type：	fibroblast, Kirsten murine sarcoma virus (Ki-MSV) transformed
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	14 to 17 days gestation embryo
Strain：	BALB/c
Storage Conditions：	liquid nitrogen vapor phase
Antigen Expression：	H-2d
Tumorigenic：	Yes
Effects：	Yes, in BALB/c mice
Comments：	They do not produce Ki-MSV.
Complete Growth Medium：	Dulbeccos modified Eagles medium, 90%; bovine calf serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Add 2.0 to 3.0 mL of Trypsin-EDTA solution (0.25% (w/v) Trypsin - 0.53mM EDTA) to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:8 Medium Renewal:xa0Twice a week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	S Aaronson
Deposited As：	Mus musculus
References：	Aaronson SA, Weaver CA. Characterization of murine sarcoma virus (Kirsten) transformation of mouse and human cells. J. Gen. Virol. 13: 245-252, 1971. PubMed: 4333712 Stephenson JR, Aaronson SA. Antigenic properties of murine sarcoma virus-transformed BALB-3T3 nonproducer cells. J. Exp. Med. 135: 503-515, 1972. PubMed: 4550769 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.