| 产品名称： | JH4 clone 1 |
|---|---|
| 商品货号： | TS211723 |
| Organism： | Cavia porcellus, guinea pig |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal female; diploid; stable |
| Clinical Data： | female |
| Complete Growth Medium： | Hams F12K medium, 90%; fetal bovine serum, 10%
|
| Subculturing： |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | R Hay |
| Deposited As： | Cavia porcellus |
| References： | Hoying J. Selection and histochemical identification of epithelial-like cells from guinea pig lung M.Sc. thesis, Wright State Univ., 1975 |
| Cross References： | Nucleotide (GenBank) : AF131949 Cavia porcellus tissue factor mRNA, complete cds. |
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| 产品名称： | JH4 clone 1 |
|---|---|
| 商品货号： | TS211723 |
| Organism： | Cavia porcellus, guinea pig |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | normal female; diploid; stable |
| Clinical Data： | female |
| Complete Growth Medium： | Hams F12K medium, 90%; fetal bovine serum, 10%
|
| Subculturing： |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | R Hay |
| Deposited As： | Cavia porcellus |
| References： | Hoying J. Selection and histochemical identification of epithelial-like cells from guinea pig lung M.Sc. thesis, Wright State Univ., 1975 |
| Cross References： | Nucleotide (GenBank) : AF131949 Cavia porcellus tissue factor mRNA, complete cds. |
