Indian Muntjac

规格:

货期:

编号:TS211749

品牌:Testobio

产品名称：	Indian Muntjac
商品货号：	TS211749
Organism：	muntjac
Tissue：	skin
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Bovine Viral Diarrhea Virus (BVDV) Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 7; range = 7 to 14. Stable karyotype in male diploid stemline number. Chromosome breaks noted in 6% of the cells examined.
Virus Susceptibility：	Herpes simplex virus Vaccinia virus Vesicular stomatitis virus Human poliovirus 1
Comments：	Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium：	Hams F10 medium, 80%; fetal bovine serum, 20%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	DH Wunsten
Deposited As：	muntjac
Year of Origin：	November, 1969
References：	Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Wurster DH, Benirschke K. Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science 168: 1364-1366, 1970. PubMed: 5444269 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

首页 > 产品中心 > 微生物培养 > 菌株 > null > Indian Muntjac

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

Indian Muntjac

货号: TS211749
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	Indian Muntjac
商品货号：	TS211749
Organism：	muntjac
Tissue：	skin
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	2 Cells contain Bovine Viral Diarrhea Virus (BVDV) Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 7; range = 7 to 14. Stable karyotype in male diploid stemline number. Chromosome breaks noted in 6% of the cells examined.
Virus Susceptibility：	Herpes simplex virus Vaccinia virus Vesicular stomatitis virus Human poliovirus 1
Comments：	Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).
Complete Growth Medium：	Hams F10 medium, 80%; fetal bovine serum, 20%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	DH Wunsten
Deposited As：	muntjac
Year of Origin：	November, 1969
References：	Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Wurster DH, Benirschke K. Indian muntjac, Muntiacus muntjak: a deer with a low diploid chromosome number. Science 168: 1364-1366, 1970. PubMed: 5444269 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.