IP2-E4

规格:

货期:

编号:TS211751

品牌:Testobio

产品名称：	IP2-E4
商品货号：	TS211751
Organism：	Mus musculus, mouse
Tissue：	axillary lymph node; vascular epithelium
Cell Type：	endothelial, SV40 transformed
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Gender：	male
Strain：	C3H/HeJ
Applications：	The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay. They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression. This clone retains the ability to differentiate on a synthetic basement-like membrane.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
Clinical Data：	male
Antigen Expression：	H-2 K; VCAM
Genes Expressed：	H-2 K; VCAM
Tumorigenic：	Yes
Effects：	Yes, the cells induced spindle tumors in nude mice with some of the histopathologic characteristics of human Kaposi Sarcoma after a shortened latency period of approximately 2 weeks
Comments：	The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). The line was cloned in 1992 by limiting dilution. IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay. The cells express the cell surface major histocompatibility complex class I antigen, H-2 k of the parental cell line. They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression. This clone retains the ability to differentiate on a synthetic basement-like membrane. The cells stain positively for SV40 T antigen.
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by Wiley-Freshney, 5th edition, published by Alan R. Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	KA OConnell
References：	OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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IP2-E4

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产品名称：	IP2-E4
商品货号：	TS211751
Organism：	Mus musculus, mouse
Tissue：	axillary lymph node; vascular epithelium
Cell Type：	endothelial, SV40 transformed
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	adult
Gender：	male
Strain：	C3H/HeJ
Applications：	The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay. They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression. This clone retains the ability to differentiate on a synthetic basement-like membrane.
Storage Conditions：	liquid nitrogen vapor phase
Derivation：	The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
Clinical Data：	male
Antigen Expression：	H-2 K; VCAM
Genes Expressed：	H-2 K; VCAM
Tumorigenic：	Yes
Effects：	Yes, the cells induced spindle tumors in nude mice with some of the histopathologic characteristics of human Kaposi Sarcoma after a shortened latency period of approximately 2 weeks
Comments：	The IP2-E4 cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161). The line was cloned in 1992 by limiting dilution. IP2-E4 cells are sensitive to lysis by activated macrophage as measured in the chromium release assay. The cells express the cell surface major histocompatibility complex class I antigen, H-2 k of the parental cell line. They do not express high levels of vascular cell adhesion molecule (VCAM) constitutively however, stimulation with TNF alpha caused an up regulation of VCAM expression. This clone retains the ability to differentiate on a synthetic basement-like membrane. The cells stain positively for SV40 T antigen.
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells, a Manual of Basic Technique by Wiley-Freshney, 5th edition, published by Alan R. Liss, N.Y., 2005.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	KA OConnell
References：	OConnell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170 OConnell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposis sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612 OConnell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposis sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.