IgH-2

规格:

货期:

编号:TS211777

品牌:Testobio

产品名称：	IgH-2
商品货号：	TS211777
Organism：	iguana
Tissue：	heart
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	immature
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 35; range = 30 to 64. The karyotype is stable. There is one large marker chromosome with a terminal centromere appearing in all cells in addition to 22 minute chromosomes, 10 large chromosomes with a median centromere and 2 large chromosomes with a submedian centromere.
Virus Susceptibility：	iguana Herpes simplex virus Pseudorabies virus Vaccinia virus Human poliovirus 1 Vesicular stomatitis virus
Complete Growth Medium：	Eagless Minimum Essential Medium with Hanks BSS, 2 mM L-glutamine, and 0.1 mM non-essential amino acids supplemented with 10% fetal bovine serum
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 36°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension into new culture vessels. Incubate cultures at 36°C in a closed flask without CO₂ in air atmosphere. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week
Cryopreservation：	Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 36°C Atmosphere: Air, 100%; no CO₂
Name of Depositor：	HF Clark
Deposited As：	iguana
Year of Origin：	August, 1966
References：	Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904 Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Cross References：	Nucleotide (GenBank) : L27530 Iguana small cytoplasmic RNA (Y3). Nucleotide (GenBank) : L27537 Iguana small cytoplasmic RNA (Y4). Nucleotide (GenBank) : L27532 Iguana small cytoplasmic RNA (Y3) gene. Nucleotide (GenBank) : L27534 Iguana small cytoplasmic RNA (Y4) gene. Nucleotide (GenBank) : L27536 Iguana small cytoplasmic RNA (Y4) gene.

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IgH-2

货号: TS211777
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产品名称：	IgH-2
商品货号：	TS211777
Organism：	iguana
Tissue：	heart
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	immature
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 35; range = 30 to 64. The karyotype is stable. There is one large marker chromosome with a terminal centromere appearing in all cells in addition to 22 minute chromosomes, 10 large chromosomes with a median centromere and 2 large chromosomes with a submedian centromere.
Virus Susceptibility：	iguana Herpes simplex virus Pseudorabies virus Vaccinia virus Human poliovirus 1 Vesicular stomatitis virus
Complete Growth Medium：	Eagless Minimum Essential Medium with Hanks BSS, 2 mM L-glutamine, and 0.1 mM non-essential amino acids supplemented with 10% fetal bovine serum
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 36°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension into new culture vessels. Incubate cultures at 36°C in a closed flask without CO₂ in air atmosphere. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week
Cryopreservation：	Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 36°C Atmosphere: Air, 100%; no CO₂
Name of Depositor：	HF Clark
Deposited As：	iguana
Year of Origin：	August, 1966
References：	Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904 Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Cross References：	Nucleotide (GenBank) : L27530 Iguana small cytoplasmic RNA (Y3). Nucleotide (GenBank) : L27537 Iguana small cytoplasmic RNA (Y4). Nucleotide (GenBank) : L27532 Iguana small cytoplasmic RNA (Y3) gene. Nucleotide (GenBank) : L27534 Iguana small cytoplasmic RNA (Y4) gene. Nucleotide (GenBank) : L27536 Iguana small cytoplasmic RNA (Y4) gene.