| 产品名称： | IGEL a2 15.3 |
|---|---|
| 商品货号： | TS211783 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP) |
| Comments： | Animals were immunized with TNP-Asc. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow cell concentration to exceed 2 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgE (Iga haplotype) |
| Name of Depositor： | M Wabl |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293 |
| Cross References： | Nucleotide (GenBank) : X65774 M.musculus DNA for IgE antibody light chain (VJ). Nucleotide (GenBank) : X65772 M.musculus DNA for IgE antibody heavy chain (VDJ). |
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| 产品名称： | IGEL a2 15.3 |
|---|---|
| 商品货号： | TS211783 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP) |
| Comments： | Animals were immunized with TNP-Asc. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow cell concentration to exceed 2 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgE (Iga haplotype) |
| Name of Depositor： | M Wabl |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293 |
| Cross References： | Nucleotide (GenBank) : X65774 M.musculus DNA for IgE antibody light chain (VJ). Nucleotide (GenBank) : X65772 M.musculus DNA for IgE antibody heavy chain (VDJ). |
