ICR-2A (RPH68.2A) [Haploid Frog]

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编号:TS211795

品牌:Testobio

产品名称：	ICR-2A (RPH68.2A) Haploid Frog
商品货号：	TS211795
Organism：	Rana pipiens, frog, grass
Tissue：	androgenetic haploid embryo
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	haploid; number 10 chromosome has a secondary constriction
Virus Susceptibility：	Frog virus 4 , Frog virus 4 Vesicular stomatitis virus Human poliovirus 1 Herpes simplex virus Vaccinia virus
Comments：	For diploid cells, see ICR 134 (ATCC CCL-128).
Complete Growth Medium：	Hams F-12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 40% double distilled water and 10% fetal bovine serum.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 25°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 25°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 25°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	J Freed, L Mezger-Freed
Deposited As：	Rana pipiens
References：	. . Methods Cell Physiol. 4: 20-46, 1970. Freed JJ, Mezger-Freed L. Stable haploid cultured cell lines from frog embryos. Proc. Natl. Acad. Sci. USA 65: 337-344, 1970. PubMed: 5263768 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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ICR-2A (RPH68.2A) [Haploid Frog]

货号: TS211795
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产品名称：	ICR-2A (RPH68.2A) Haploid Frog
商品货号：	TS211795
Organism：	Rana pipiens, frog, grass
Tissue：	androgenetic haploid embryo
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	embryo
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	haploid; number 10 chromosome has a secondary constriction
Virus Susceptibility：	Frog virus 4 , Frog virus 4 Vesicular stomatitis virus Human poliovirus 1 Herpes simplex virus Vaccinia virus
Comments：	For diploid cells, see ICR 134 (ATCC CCL-128).
Complete Growth Medium：	Hams F-12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 40% double distilled water and 10% fetal bovine serum.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 25°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 25°C. Subcultivation Ratio: 1:2 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a Manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium 95%; DMSO, 5%.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 25°C Atmosphere: Air, 95%; CO₂, 5%
Name of Depositor：	J Freed, L Mezger-Freed
Deposited As：	Rana pipiens
References：	. . Methods Cell Physiol. 4: 20-46, 1970. Freed JJ, Mezger-Freed L. Stable haploid cultured cell lines from frog embryos. Proc. Natl. Acad. Sci. USA 65: 337-344, 1970. PubMed: 5263768 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.