Hybridoma 231
Hybridoma 231
规格:
货期:
编号:TS211806
品牌:Testobio
| 产品名称： | Hybridoma 231 |
|---|---|
| 商品货号： | TS211806 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes. It cross-reacts with human granulocytes. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against canine lymphoma cells |
| Cellular Products： | immunoglobulin; monoclonal antibody; against canine lymphoma cells |
| Comments： | Animals were immunized with canine lymphoma cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes. It cross-reacts with human granulocytes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | Wistar Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Steplewski Z, Jeglum KA. Monoclonal antibodies against lymphoma-associated antigens, hybrid cell lines producing these antibodies. US Patent 5,169,775 dated Dec 8 1992 |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
Hybridoma 231
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | Hybridoma 231 |
|---|---|
| 商品货号： | TS211806 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes. It cross-reacts with human granulocytes. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against canine lymphoma cells |
| Cellular Products： | immunoglobulin; monoclonal antibody; against canine lymphoma cells |
| Comments： | Animals were immunized with canine lymphoma cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes. It cross-reacts with human granulocytes. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | Wistar Institute |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Steplewski Z, Jeglum KA. Monoclonal antibodies against lymphoma-associated antigens, hybrid cell lines producing these antibodies. US Patent 5,169,775 dated Dec 8 1992 |
