| 产品名称： | HT |
|---|---|
| 商品货号： | TS211820 |
| Organism： | Homo sapiens, human |
| Tissue： | ascites |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | diffuse mixed lymphoma |
| Age： | 70 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo. |
| Clinical Data： | 70 years Caucasian male |
| Antigen Expression： | CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) - |
| Genes Expressed： | immunoglobulin; kappa light chain |
| Cellular Products： | immunoglobulin; kappa light chain |
| Comments： | HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface.
Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
HT cells have been reported to be Epstein-Barr virus genome negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL. Medium Renewal: 2 to 3 times a week. xa0 |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 13,14 D16S539: 11,13 D5S818: 11,13 D7S820: 8,10 THO1: 6,7 TPOX: 11 vWA: 17,18 |
| Isotype： | kappa |
| Name of Depositor： | M Beckwith |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1983 |
| References： | Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723 |
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| 产品名称： | HT |
|---|---|
| 商品货号： | TS211820 |
| Organism： | Homo sapiens, human |
| Tissue： | ascites |
| Cell Type： | B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | diffuse mixed lymphoma |
| Age： | 70 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo. |
| Clinical Data： | 70 years Caucasian male |
| Antigen Expression： | CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) - |
| Genes Expressed： | immunoglobulin; kappa light chain |
| Cellular Products： | immunoglobulin; kappa light chain |
| Comments： | HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface.
Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
HT cells have been reported to be Epstein-Barr virus genome negative. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL. Medium Renewal: 2 to 3 times a week. xa0 |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10 D13S317: 13,14 D16S539: 11,13 D5S818: 11,13 D7S820: 8,10 THO1: 6,7 TPOX: 11 vWA: 17,18 |
| Isotype： | kappa |
| Name of Depositor： | M Beckwith |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1983 |
| References： | Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723 |
