HT-1080 [HT1080]

规格:

货期:

编号:TS211826

品牌:Testobio

产品名称：	HT-1080 HT1080
商品货号：	TS211826
Organism：	Homo sapiens, human
Tissue：	connective tissue
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	fibrosarcoma
Age：	35 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 46; range = 44 to 48. Pseudodiploidy was frequently noted. About 40% of the cells had rearranged karyotypes with an extra E-group chromosome and a group C chromosome, probably chromosome 11, was missing. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images：
Clinical Data：	35 years Caucasian male
Oncogene：	ras +
Tumorigenic：	Yes
Effects：	Yes, in immunosuppressed mice
Virus Susceptibility：	Human poliovirus 1 RD-114 Feline Feline leukemia virus Vesicular stomatitis virus
Comments：	The cells contain an activated N-ras oncogene.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 12 D13S317: 12,14 D16S539: 9,12 D5S818: 11,13 D7S820: 9,10 THO1: 6 TPOX: 8 vWA: 14,19
Isoenzymes：	G6PD, B
Year of Origin：	July, 1972
References：	Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 Geiser AG, et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing different activated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. PubMed: 2647289 Rasheed S, et al. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 33: 1027-1033, 1974. PubMed: 4132053 Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716 Proc. Am. Assoc. Cancer Res. 8: 1, 1967. Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031 Iida A, et al. Inducible gene expression by retrovirus-mediated transfer of a modified tetracycline-regulated system. J. Virol. 70: 6054-6059, 1996. PubMed: 8709228 Brenneman M, et al. Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc. Natl. Acad. Sci. USA 93: 3608-3612, 1996. PubMed: 8622983 Seiffert D. Hydrolysis of platelet vitronectin by calpain. J. Biol. Chem. 271: 11170-11176, 1996. PubMed: 8626663 Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651
Cross References：	Nucleotide (GenBank) : X85129 H.sapiens mRNA for humer. Nucleotide (GenBank) : X82321 H.sapiens mRNA for thiol-specific antioxidant.

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HT-1080 [HT1080]

货号: TS211826
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产品名称：	HT-1080 HT1080
商品货号：	TS211826
Organism：	Homo sapiens, human
Tissue：	connective tissue
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	fibrosarcoma
Age：	35 years
Gender：	male
Ethnicity：	Caucasian
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 46; range = 44 to 48. Pseudodiploidy was frequently noted. About 40% of the cells had rearranged karyotypes with an extra E-group chromosome and a group C chromosome, probably chromosome 11, was missing. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Images：
Clinical Data：	35 years Caucasian male
Oncogene：	ras +
Tumorigenic：	Yes
Effects：	Yes, in immunosuppressed mice
Virus Susceptibility：	Human poliovirus 1 RD-114 Feline Feline leukemia virus Vesicular stomatitis virus
Comments：	The cells contain an activated N-ras oncogene.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 12 D13S317: 12,14 D16S539: 9,12 D5S818: 11,13 D7S820: 9,10 THO1: 6 TPOX: 8 vWA: 14,19
Isoenzymes：	G6PD, B
Year of Origin：	July, 1972
References：	Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 Geiser AG, et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing different activated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. PubMed: 2647289 Rasheed S, et al. Characterization of a newly derived human sarcoma cell line (HT-1080). Cancer 33: 1027-1033, 1974. PubMed: 4132053 Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716 Proc. Am. Assoc. Cancer Res. 8: 1, 1967. Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031 Iida A, et al. Inducible gene expression by retrovirus-mediated transfer of a modified tetracycline-regulated system. J. Virol. 70: 6054-6059, 1996. PubMed: 8709228 Brenneman M, et al. Stimulation of intrachromosomal homologous recombination in human cells by electroporation with site-specific endonucleases. Proc. Natl. Acad. Sci. USA 93: 3608-3612, 1996. PubMed: 8622983 Seiffert D. Hydrolysis of platelet vitronectin by calpain. J. Biol. Chem. 271: 11170-11176, 1996. PubMed: 8626663 Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651
Cross References：	Nucleotide (GenBank) : X85129 H.sapiens mRNA for humer. Nucleotide (GenBank) : X82321 H.sapiens mRNA for thiol-specific antioxidant.