Hs 929.Sk
Hs 929.Sk
规格:
货期:
编号:TS211829
品牌:Testobio
| 产品名称： | Hs 929.Sk |
|---|---|
| 商品货号： | TS211829 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | flask |
| Morphology： | mixed |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Granuloma |
| Age： | 5 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 46 |
| Images： |  |
| Derivation： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Clinical Data： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). male Caucasian 5 years |
| Comments： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. Note: This item is distributed only within the 50 United States. It is not available for international distribution. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Passage History： | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
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章丽敏
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朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | Hs 929.Sk |
|---|---|
| 商品货号： | TS211829 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Product Format： | flask |
| Morphology： | mixed |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Granuloma |
| Age： | 5 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 46 |
| Images： | |
| Derivation： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
| Clinical Data： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). male Caucasian 5 years |
| Comments： | The line was established from apparently normal tissue from a patient who had eosinophilic granuloma (see ATCC CRL-7682). Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. Note: This item is distributed only within the 50 United States. It is not available for international distribution. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Passage History： | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC. We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. |
