Hs 683

规格:

货期:

编号:TS211901

品牌:Testobio

产品名称：	Hs 683
商品货号：	TS211901
Organism：	Homo sapiens, human
Tissue：	brain
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	glioma
Age：	76 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 82; range = 74 to 87. This is a hypertriploid human cell line with the modal chromosome number of 82 occurring in 16% of cells, with the distribution skewed toward the lower number range. Higher ploidies occurred in 3.0% of cells. Most cells had 4-6 marker chromosomes, but only del(3) (q12.2q21) occurred frequently in most cells, and der(9)t(9;?) (q34.2;?), tdic(1;4) (q21;q35), and a minute acrocentric occurred in more than two cells. N7 occurred in 4-6 copies per cell. Two X and one or two Y chromosomes were present in most cells.
Derivation：	Hs 683 was isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian.
Clinical Data：	76 years Caucasian male
Tumorigenic：	No
Effects：	No, in immunosuppressed mice Yes, in semisolid medium
Comments：	Microvilli, but no desmosomes were observed.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: Twice per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 9,13 D13S317: 8,12 D16S539: 9,10 D5S818: 11,12 D7S820: 11 THO1: 6,8 TPOX: 8,11 vWA: 18,20
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1-2
Name of Depositor：	RB Owens
Deposited As：	Homo sapiens
References：	Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406
Cross References：	Nucleotide (GenBank) : M84526 Human adipsin/complement factor D mRNA, complete cds.

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Hs 683

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产品名称：	Hs 683
商品货号：	TS211901
Organism：	Homo sapiens, human
Tissue：	brain
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	glioma
Age：	76 years
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 82; range = 74 to 87. This is a hypertriploid human cell line with the modal chromosome number of 82 occurring in 16% of cells, with the distribution skewed toward the lower number range. Higher ploidies occurred in 3.0% of cells. Most cells had 4-6 marker chromosomes, but only del(3) (q12.2q21) occurred frequently in most cells, and der(9)t(9;?) (q34.2;?), tdic(1;4) (q21;q35), and a minute acrocentric occurred in more than two cells. N7 occurred in 4-6 copies per cell. Two X and one or two Y chromosomes were present in most cells.
Derivation：	Hs 683 was isolated from explant cultures of a glioma taken from the left temporal lobe of a 76 year old male Caucasian.
Clinical Data：	76 years Caucasian male
Tumorigenic：	No
Effects：	No, in immunosuppressed mice Yes, in semisolid medium
Comments：	Microvilli, but no desmosomes were observed.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended Medium Renewal: Twice per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 9,13 D13S317: 8,12 D16S539: 9,10 D5S818: 11,12 D7S820: 11 THO1: 6,8 TPOX: 8,11 vWA: 18,20
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1 PGM3, 1-2
Name of Depositor：	RB Owens
Deposited As：	Homo sapiens
References：	Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033 Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406
Cross References：	Nucleotide (GenBank) : M84526 Human adipsin/complement factor D mRNA, complete cds.