| 产品名称： | HP6014 |
|---|---|
| 商品货号： | TS211981 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region. This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with purified human immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with purified human immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region. This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 10 mM HEPES and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | CB Reimer |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201 Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923 |
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13372109301
| 产品名称： | HP6014 |
|---|---|
| 商品货号： | TS211981 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region. This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with purified human immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region) |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with purified human immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region. This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 10 mM HEPES and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | CB Reimer |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201 Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923 |
