HMCB [ Human Melanoma Cell Bowes]

规格:

货期:

编号:TS211994

品牌:Testobio

产品名称：	HMCB Human Melanoma Cell Bowes
商品货号：	TS211994
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	melanoma
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed：	human tissue plasminogen activator
Cellular Products：	human tissue plasminogen activator
Comments：	The cells originally tested positive for mycoplasma. After treatment with ciprofloxacin, the cells have remained negative for mycoplasma for 60 days.
Complete Growth Medium：	Minimum essential medium (Eagle) with non-essential amino acids, 10 mM HEPES and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Two to three times weekly
Cryopreservation：	Freeze medium: culture medium 95%; DMSO 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Name of Depositor：	Genentech, Inc.
Deposited As：	Homo sapiens
U.S. Patent Number：	4,766,075

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HMCB [ Human Melanoma Cell Bowes]

货号: TS211994
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产品名称：	HMCB Human Melanoma Cell Bowes
商品货号：	TS211994
Organism：	Homo sapiens, human
Tissue：	skin
Cell Type：	epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	melanoma
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Disclosure：	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed：	human tissue plasminogen activator
Cellular Products：	human tissue plasminogen activator
Comments：	The cells originally tested positive for mycoplasma. After treatment with ciprofloxacin, the cells have remained negative for mycoplasma for 60 days.
Complete Growth Medium：	Minimum essential medium (Eagle) with non-essential amino acids, 10 mM HEPES and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing：	Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Two to three times weekly
Cryopreservation：	Freeze medium: culture medium 95%; DMSO 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Name of Depositor：	Genentech, Inc.
Deposited As：	Homo sapiens
U.S. Patent Number：	4,766,075