HFL1

规格:

货期:

编号:TS212008

品牌:Testobio

产品名称：	HFL1
商品货号：	TS212008
Organism：	Homo sapiens, human
Tissue：	lung
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	fetus
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	normal human; diploid; stable Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Clinical Data：	Caucasian fetus
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C.to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio:xa0A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal:xa02 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 7,9 TPOX: 6,9 vWA: 17
Isoenzymes：	G6PD, B
Name of Depositor：	RG Crystal
Deposited As：	Homo sapiens
References：	Breul SD, et al. Control of collagen production by human diploid lung fibroblasts. J. Biol. Chem. 255: 5250-5260, 1980. PubMed: 7372635
Cross References：	Nucleotide (GenBank) : X06700 Human mRNA 3 region for pro-alpha1(III) collagen. Nucleotide (GenBank) : X06700 Human mRNA 3 region for pro-alpha1(III) collagen.

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HFL1

货号: TS212008
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产品名称：	HFL1
商品货号：	TS212008
Organism：	Homo sapiens, human
Tissue：	lung
Cell Type：	fibroblast
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	fetus
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	normal human; diploid; stable Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.
Clinical Data：	Caucasian fetus
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing：	Volumes used in this protocol are for a 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).xa0Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.xa0 Cells that are difficult to detach may be placed at 37°C.to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.xa0 Add appropriate aliquots of the cell suspension to new culture vessels.xa0 Incubate cultures at 37°C. Subcultivation Ratio:xa0A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal:xa02 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
STR Profile：	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,12 D16S539: 9,11 D5S818: 12 D7S820: 9,10 THO1: 7,9 TPOX: 6,9 vWA: 17
Isoenzymes：	G6PD, B
Name of Depositor：	RG Crystal
Deposited As：	Homo sapiens
References：	Breul SD, et al. Control of collagen production by human diploid lung fibroblasts. J. Biol. Chem. 255: 5250-5260, 1980. PubMed: 7372635
Cross References：	Nucleotide (GenBank) : X06700 Human mRNA 3 region for pro-alpha1(III) collagen. Nucleotide (GenBank) : X06700 Human mRNA 3 region for pro-alpha1(III) collagen.