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HCC2157
HCC2157
规格:
货期:
编号:TS212043
品牌:Testobio
产品名称: HCC2157
商品货号: TS212043
Organism: Homo sapiens, human
Tissue: mammary gland; breast
Cell Type: Epithelial
Product Format: frozen
Morphology: epithelial
Culture Properties: suspension, multicell aggregates, Cells in suspension show an inclination to form duct-like structures and gland-like structures.
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: TNM stage IIIA, grade 2,primary ductal carcinoma
Age: 48 years adult
Gender: female
Ethnicity: Black
Applications:
HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +).
The cells are positive for expression of Her2-neu and for expression of p53.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
Storage Conditions: liquid nitrogen vapor temperature
Karyotype: Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Images: Cell Micrograph of TS212043, HCC2157
Derivation:
This cell line was initiated on 3/4/96 and took 8 months to establish.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341.
Clinical Data:
Black
female
48 years

Receptor Expression:
estrogen receptor, negative
progesterone receptor, positive
Oncogene: her2/neu +, p53 +
Genes Expressed:
Epithelial glycoprotein 2 EGP2; cytokeratin 19
Cellular Products:
Epithelial glycoprotein 2 EGP2; cytokeratin 19
Comments:
The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341.
Complete Growth Medium: RPMI-1640 medium, 95%; fetal bovine serum, 5%
Subculturing: Cultures can be maintained by addition of fresh medium. Cultures grow as floating aggregates of large irregular cell clusters.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation:
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions:
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile:
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 13
D7S820: 10,12
THO1: 8,9
TPOX: 9,11
vWA: 14,18
Name of Depositor: AF Gazdar, AK Virmani
Year of Origin: 1996
References:

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

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HCC2157

  • 货号: TS212043
  • 好评
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  • 品牌 : TESTOBIO
产品名称: HCC2157
商品货号: TS212043
Organism: Homo sapiens, human
Tissue: mammary gland; breast
Cell Type: Epithelial
Product Format: frozen
Morphology: epithelial
Culture Properties: suspension, multicell aggregates, Cells in suspension show an inclination to form duct-like structures and gland-like structures.
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: TNM stage IIIA, grade 2,primary ductal carcinoma
Age: 48 years adult
Gender: female
Ethnicity: Black
Applications:
HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +).
The cells are positive for expression of Her2-neu and for expression of p53.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
Storage Conditions: liquid nitrogen vapor temperature
Karyotype: Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%
Images: Cell Micrograph of TS212043, HCC2157
Derivation:
This cell line was initiated on 3/4/96 and took 8 months to establish.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341.
Clinical Data:
Black
female
48 years

Receptor Expression:
estrogen receptor, negative
progesterone receptor, positive
Oncogene: her2/neu +, p53 +
Genes Expressed:
Epithelial glycoprotein 2 EGP2; cytokeratin 19
Cellular Products:
Epithelial glycoprotein 2 EGP2; cytokeratin 19
Comments:
The tumor was classified as TNM stage IIIA, grade 2, ductal carcinoma with 1 out of 9 lymph node metastasis.
The cells form ducts and morula-like structures (hollow spherical structures lined with a single layer of cells).
HCC2157 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.
An EBV transformed lymphoblastoid cell line (HCC2157 BL) from the same patient is available as ATCC CRL-2341.
Complete Growth Medium: RPMI-1640 medium, 95%; fetal bovine serum, 5%
Subculturing: Cultures can be maintained by addition of fresh medium. Cultures grow as floating aggregates of large irregular cell clusters.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation:
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions:
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile:
Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 11
D5S818: 13
D7S820: 10,12
THO1: 8,9
TPOX: 9,11
vWA: 14,18
Name of Depositor: AF Gazdar, AK Virmani
Year of Origin: 1996
References:

Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

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