HCC70

规格:

货期:

编号:TS212045

品牌:Testobio

产品名称：	HCC70
商品货号：	TS212045
Organism：	Homo sapiens, human
Tissue：	mammary gland; breast/duct
Cell Type：	Epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent, The line grows as attached medium-sized epithelial cells without floating cells.
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	TNM stage IIIA, grade 3,primary ductal carcinoma
Age：	49 years
Gender：	female
Ethnicity：	Black
Applications：	HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	polyploid
Images：
Derivation：	The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.
Clinical Data：	49 years Black female
Receptor Expression：	progesterone receptor, not expressed
Oncogene：	her2/neu -, p53 + (overexpressed)
Genes Expressed：	Epithelial glycoprotein 2 EGP2; cytokeratin 19,her2/neu -, p53 + (overexpressed),The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.,The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Cellular Products：	Epithelial glycoprotein 2 EGP2; cytokeratin 19
Comments：	The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish. The cells are poorly differentiated. The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 17 lymph nodes. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. An independent study, by ATCC Scientists, is underway to assess the estrogen receptor status; results of this study will be published online upon completion.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
STR Profile：	Amelogenin:X CSF1PO:10,14 D13S317:12 D16S539:9,13 D5S818:12,13 D7S820:10,11 THO1:9 TPOX:10 vWA: 13,15
Name of Depositor：	AF Gazdar, AK Virmani
Deposited As：	Homo sapiens
Year of Origin：	1992
References：	Kao J, et al. Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery. PlosONE 4 (7): e 6146, 2009 Kim MS, et al. Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform. PlosONE 5 (5): e10441, 2010 Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771

首页 > 产品中心 > 微生物培养 > 菌株 > null > HCC70

产品中心 Product Center

联系我们CONTACT US

0574-87917803
24小时服务热线
testobio@163.com
销售邮箱
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址

联系方式

章丽敏
17757489010
朱静
18067106081
李晴
18067408036
李晶晶
13375187189
周雪
13372109301

HCC70

货号: TS212045
好评

询价

品牌 : TESTOBIO

加入购物车在线咨询

产品名称：	HCC70
商品货号：	TS212045
Organism：	Homo sapiens, human
Tissue：	mammary gland; breast/duct
Cell Type：	Epithelial
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent, The line grows as attached medium-sized epithelial cells without floating cells.
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	TNM stage IIIA, grade 3,primary ductal carcinoma
Age：	49 years
Gender：	female
Ethnicity：	Black
Applications：	HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	polyploid
Images：
Derivation：	The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish.
Clinical Data：	49 years Black female
Receptor Expression：	progesterone receptor, not expressed
Oncogene：	her2/neu -, p53 + (overexpressed)
Genes Expressed：	Epithelial glycoprotein 2 EGP2; cytokeratin 19,her2/neu -, p53 + (overexpressed),The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes.,The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
Cellular Products：	Epithelial glycoprotein 2 EGP2; cytokeratin 19
Comments：	The HCC70 cell line was initiated from a primary ductal carcinoma on June 3, 1992, and took 44 months to establish. The cells are poorly differentiated. The tumor was classified as TNM Stage IIIA, grade 3, invasive ductal carcinoma with metastases in 4 out of 17 lymph nodes. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. An independent study, by ATCC Scientists, is underway to assess the estrogen receptor status; results of this study will be published online upon completion.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.xa0Corning^® T-75 flasks (catalog #430641) are recommended for subculturing this product. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
STR Profile：	Amelogenin:X CSF1PO:10,14 D13S317:12 D16S539:9,13 D5S818:12,13 D7S820:10,11 THO1:9 TPOX:10 vWA: 13,15
Name of Depositor：	AF Gazdar, AK Virmani
Deposited As：	Homo sapiens
Year of Origin：	1992
References：	Kao J, et al. Molecular profiling of breast cancer cell lines defines relevant tumor models and provides a resource for cancer gene discovery. PlosONE 4 (7): e 6146, 2009 Kim MS, et al. Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform. PlosONE 5 (5): e10441, 2010 Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771