HaK

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货期:

编号:TS212075

品牌:Testobio

产品名称：	HaK
商品货号：	TS212075
Organism：	Mesocricetus auratus, hamster, Syrian golden
Tissue：	kidney
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Tumorigenic：	Yes
Effects：	Yes, in hamsters (Tumors developed in 5/9 hamsters injected intra-cerebrally with 0.03 ml containing 7000 to 15000 cells (Moore, AE, personal communication).)
Virus Resistance：	poliovirus 1, 2, 3; adenovirus 3; coxsackievirus A4, A8, B1; herpes simplex; smallpox (variola); influenzaviruses (Asian)
Complete Growth Medium：	Complete Growth Medium The base medium for this cell line is Eagles Minimum Essential Medium (ATCC® No.xa030-2003).xa0 To make the complete growth medium, add the following components to the base medium:xa0 Calf Serum (ATCC® No.xa030-2030)xa0to a final concentration of 10%. This medium is formulated for use in 5% CO₂.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37ºC to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: 5%xa0CO₂
Name of Depositor：	AE Moore
Deposited As：	Mesocricetus auratus

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HaK

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产品名称：	HaK
商品货号：	TS212075
Organism：	Mesocricetus auratus, hamster, Syrian golden
Tissue：	kidney
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	adult
Gender：	male
Storage Conditions：	liquid nitrogen vapor phase
Tumorigenic：	Yes
Effects：	Yes, in hamsters (Tumors developed in 5/9 hamsters injected intra-cerebrally with 0.03 ml containing 7000 to 15000 cells (Moore, AE, personal communication).)
Virus Resistance：	poliovirus 1, 2, 3; adenovirus 3; coxsackievirus A4, A8, B1; herpes simplex; smallpox (variola); influenzaviruses (Asian)
Complete Growth Medium：	Complete Growth Medium The base medium for this cell line is Eagles Minimum Essential Medium (ATCC® No.xa030-2003).xa0 To make the complete growth medium, add the following components to the base medium:xa0 Calf Serum (ATCC® No.xa030-2030)xa0to a final concentration of 10%. This medium is formulated for use in 5% CO₂.
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37ºC to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in *Culture of Animal Cells, a manual of Basic Technique* by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: 5%xa0CO₂
Name of Depositor：	AE Moore
Deposited As：	Mesocricetus auratus