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H25B10
H25B10
规格:
货期:
编号:TS212083
品牌:Testobio
产品名称: H25B10
商品货号: TS212083
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: hepatitis
Applications:
This is a new deposit of ATCC CRL-8017 that secretes greater amounts of immunoglobulin.
Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation:
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Cellular Products:
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Comments:
Animals were immunized with hepatitis B virus surface antigen.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
This is a new deposit of ATCC CRL-8017 that secretes greater amounts of immunoglobulin.
Complete Growth Medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 85%; fetal bovine serum, 15%
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: Massachusetts General Hospital
Deposited As: mouse (B cell); mouse (myeloma)
References:

Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981

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H25B10

  • 货号: TS212083
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询价
  • 品牌 : TESTOBIO
产品名称: H25B10
商品货号: TS212083
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease: hepatitis
Applications:
This is a new deposit of ATCC CRL-8017 that secretes greater amounts of immunoglobulin.
Disclosure: This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation:
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Cellular Products:
immunoglobulin; monoclonal antibody; against hepatitis B virus surface antigen (HBsAg)
Comments:
Animals were immunized with hepatitis B virus surface antigen.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
This is a new deposit of ATCC CRL-8017 that secretes greater amounts of immunoglobulin.
Complete Growth Medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 85%; fetal bovine serum, 15%
Subculturing:
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype: IgG1
Name of Depositor: Massachusetts General Hospital
Deposited As: mouse (B cell); mouse (myeloma)
References:

Wands JR, Zurawski VR Jr.. Process for producing antibodies to hepatitis virus and cell lines therefor. US Patent 4,271,145 dated Jun 2 1981

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