你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: call_new 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
GS-109-V-34
GS-109-V-34
规格:
货期:
编号:TS212089
品牌:Testobio
产品名称： GS-109-V-34
商品货号： TS212089
Organism： Homo sapiens, human
Product Format： frozen
Morphology： fibroblast
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： Adenoma
Age： 6 years
Gender： female
Ethnicity： Caucasian
Storage Conditions： liquid nitrogen vapor phase
Karyotype： This cell line has the normal female karyotype, 46,XX. The modal chromosome number was 46, occurring in 84% of cells, and the rate of polyploidy was 2.8%.The X chromosome was paired. There were no consistent aberrations in all analyzed cells.
Clinical Data：
The tissue donor suffered from Gardners syndrome, a form of Familial adenomatous polyposis (FAP).
female
Caucasian
6 years
Tumorigenic： No
Effects：
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments：
The tissue donor suffered from Gardners syndrome, a form of Familial adenomatous polyposis (FAP). It is an autosomal dominant condition with predisposition to carcinoma and multiple polyps of the colon.
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation：
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37.0°C
Name of Depositor： EJ Gardner
Deposited As： Homo sapiens
首页 > 产品中心 > 微生物培养 > 菌株 > null > GS-109-V-34

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

GS-109-V-34

  • 货号: TS212089
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： GS-109-V-34
商品货号： TS212089
Organism： Homo sapiens, human
Product Format： frozen
Morphology： fibroblast
Culture Properties： adherent
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease： Adenoma
Age： 6 years
Gender： female
Ethnicity： Caucasian
Storage Conditions： liquid nitrogen vapor phase
Karyotype： This cell line has the normal female karyotype, 46,XX. The modal chromosome number was 46, occurring in 84% of cells, and the rate of polyploidy was 2.8%.The X chromosome was paired. There were no consistent aberrations in all analyzed cells.
Clinical Data：
The tissue donor suffered from Gardners syndrome, a form of Familial adenomatous polyposis (FAP).
female
Caucasian
6 years
Tumorigenic： No
Effects：
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments：
The tissue donor suffered from Gardners syndrome, a form of Familial adenomatous polyposis (FAP). It is an autosomal dominant condition with predisposition to carcinoma and multiple polyps of the colon.
Complete Growth Medium： The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing：
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation：
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions：
Temperature: 37.0°C
Name of Depositor： EJ Gardner
Deposited As： Homo sapiens
testopic
小红书
扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: