GH3

规格:

货期:

编号:TS212115

品牌:Testobio

产品名称：	GH3
商品货号：	TS212115
Organism：	Rattus norvegicus, rat
Tissue：	pituitary
Cell Type：	Epithelial-like
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	loosely adherent with floating clusters
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor
Age：	7 months
Gender：	female
Strain：	Wistar-Furth
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 67; range = 47 to 71. Stemline karyotype is stable with a few structural alterations. Two dicentric marker chromosomes were observed in 100% of the cells examined.
Derivation：	Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established. Clone GH3 was established in July, 1965 by A.H. Tashjian, Jr., et al. from a pituitary tumor carried in a 7-month-old female Wistar-Furth rat.
Clinical Data：	female 7 months
Genes Expressed：	prolactin; growth hormone (somatotrophin)
Cellular Products：	prolactin; growth hormone (somatotrophin)
Virus Susceptibility：	Herpes simplex virus Vesicular stomatitis virus Human poliovirus 1
Comments：	The epithelial-like GH3 clone generates growth hormone at a greater rate than the GH1 cells and also produces prolactin. Studies on the control of the production of these protein hormones by the GH3 cells have shown that hydrocortisone stimulates the production of growth hormone and inhibits prolactin production. The cells have been adapted to growth in suspension culture using Eagles minimum essential medium (spinner) supplemented with 15% horse serum and 2.5% FBS. Under these conditions the cells continue to produce both growth hormone and prolactin
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Name of Depositor：	AH Tashjian
Deposited As：	Rattus sp.
Passage History：	Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established. The cell line was submitted to the American Type Culture Collection in October, 1972 in the 10th subculture generation. xa0
Year of Origin：	July, 1965
References：	Bancroft FC, et al. Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone. J. Cell Biol. 43: 432-441, 1969. PubMed: 5389137 Tashjian AH Jr., et al. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281 Tashjian AH Jr., et al. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells. J. Cell Biol. 47: 61-70, 1970. PubMed: 5513559 Bancroft FC, Tashjian AH Jr.. Growth in suspension culture of rat pituitary cells which produce growth hormone and prolactin. Exp. Cell Res. 64: 125-128, 1971. PubMed: 5541958 Bancroft FC, Tashjian AH Jr.. Control of the production of two protein hormones by rat pituitary cells in culture. In Vitro 6: 180-189, 1970. PubMed: 5535574 Wood WM, et al. Thyroid hormone receptor beta2 promoter activity in pituitary cells is regulated by Pit-1. J. Biol. Chem. 271: 24213-24220, 1996. PubMed: 8798664

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GH3

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产品名称：	GH3
商品货号：	TS212115
Organism：	Rattus norvegicus, rat
Tissue：	pituitary
Cell Type：	Epithelial-like
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	loosely adherent with floating clusters
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	tumor
Age：	7 months
Gender：	female
Strain：	Wistar-Furth
Applications：	This cell line is a suitable transfection host.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 67; range = 47 to 71. Stemline karyotype is stable with a few structural alterations. Two dicentric marker chromosomes were observed in 100% of the cells examined.
Derivation：	Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established. Clone GH3 was established in July, 1965 by A.H. Tashjian, Jr., et al. from a pituitary tumor carried in a 7-month-old female Wistar-Furth rat.
Clinical Data：	female 7 months
Genes Expressed：	prolactin; growth hormone (somatotrophin)
Cellular Products：	prolactin; growth hormone (somatotrophin)
Virus Susceptibility：	Herpes simplex virus Vesicular stomatitis virus Human poliovirus 1
Comments：	The epithelial-like GH3 clone generates growth hormone at a greater rate than the GH1 cells and also produces prolactin. Studies on the control of the production of these protein hormones by the GH3 cells have shown that hydrocortisone stimulates the production of growth hormone and inhibits prolactin production. The cells have been adapted to growth in suspension culture using Eagles minimum essential medium (spinner) supplemented with 15% horse serum and 2.5% FBS. Under these conditions the cells continue to produce both growth hormone and prolactin
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing：	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
Cryopreservation：	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Name of Depositor：	AH Tashjian
Deposited As：	Rattus sp.
Passage History：	Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established. The cell line was submitted to the American Type Culture Collection in October, 1972 in the 10th subculture generation. xa0
Year of Origin：	July, 1965
References：	Bancroft FC, et al. Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone. J. Cell Biol. 43: 432-441, 1969. PubMed: 5389137 Tashjian AH Jr., et al. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281 Tashjian AH Jr., et al. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells. J. Cell Biol. 47: 61-70, 1970. PubMed: 5513559 Bancroft FC, Tashjian AH Jr.. Growth in suspension culture of rat pituitary cells which produce growth hormone and prolactin. Exp. Cell Res. 64: 125-128, 1971. PubMed: 5541958 Bancroft FC, Tashjian AH Jr.. Control of the production of two protein hormones by rat pituitary cells in culture. In Vitro 6: 180-189, 1970. PubMed: 5535574 Wood WM, et al. Thyroid hormone receptor beta2 promoter activity in pituitary cells is regulated by Pit-1. J. Biol. Chem. 271: 24213-24220, 1996. PubMed: 8798664