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GAP A3
GAP A3
规格:
货期:
编号:TS212120
品牌:Testobio
产品名称: GAP A3
商品货号: TS212120
Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type: hybridoma: B lymphocyte
Product Format: frozen
Morphology: lymphoblast
Culture Properties: suspension, suspension
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications:
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions: liquid nitrogen vapor phase
Derivation:
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed:
immunoglobulin; monoclonal antibody; against HLA A3
Cellular Products:
immunoglobulin; monoclonal antibody; against HLA A3
Comments:
Animals were immunized with human peripheral blood lymphocytes.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with HLA A11.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 1 X 10(5) viable cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation:
    Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Isotype: IgG2a; kappa light chain
    Name of Depositor: AE Berger
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    . . Fed. Proc. 42: 1371, 1983.

    Dieckmann CL, et al. Monoclonal antibody to HLA-A3. Hybridoma 1: 87-90, 1982. PubMed: 6208124

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    GAP A3

    • 货号: TS212120
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称: GAP A3
    商品货号: TS212120
    Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
    Cell Type: hybridoma: B lymphocyte
    Product Format: frozen
    Morphology: lymphoblast
    Culture Properties: suspension, suspension
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Applications:
    Tested and found negative for ectromelia virus (mousepox).
    Storage Conditions: liquid nitrogen vapor phase
    Derivation:
    Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
    Genes Expressed:
    immunoglobulin; monoclonal antibody; against HLA A3
    Cellular Products:
    immunoglobulin; monoclonal antibody; against HLA A3
    Comments:
    Animals were immunized with human peripheral blood lymphocytes.
    Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
    The antibody does not react with HLA A11.
    Tested and found negative for ectromelia virus (mousepox).
    Complete Growth Medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
  • Subculturing:
    Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 1 X 10(5) viable cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml.
    Medium Renewal: Every 2 to 3 days
    Cryopreservation:
    Freeze medium: culture medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Isotype: IgG2a; kappa light chain
    Name of Depositor: AE Berger
    Deposited As: mouse (B cell); mouse (myeloma)
    References:

    . . Fed. Proc. 42: 1371, 1983.

    Dieckmann CL, et al. Monoclonal antibody to HLA-A3. Hybridoma 1: 87-90, 1982. PubMed: 6208124

    合作单位: