| 产品名称： | G-401 G401 |
|---|---|
| 商品货号： | TS212129 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | rhabdoid tumor (formerly classified as Wilms tumor) |
| Age： | 3 months |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | The cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation. |
| Images： |  |
| Derivation： | G-401 was deposited as a cell line derived from a Wilms tumor. |
| Clinical Data： | 3 months Caucasian male |
| Genes Expressed： | nephroblast growth factor (NB-GF) |
| Cellular Products： | nephroblast growth factor (NB-GF) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms colonies in soft agar |
| Comments： | G-401 was deposited as a cell line derived from a Wilms tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. Ref   Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 9,14 D16S539: 12 D5S818: 13 D7S820: 11,14 THO1: 8,9.3 TPOX: 8,11 vWA: 16 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | PT Peebles |
| Deposited As： | Homo sapiens |
| References： | Weissman BE, et al. Introduction of a normal human chromosome 11 into a Wilms tumor cell line controls its tumorigenic expression. Science 236: 175-176, 1987. PubMed: 3031816 Burrow CR, Wilson PD. A putative Wilms tumor-secreted growth factor activity required for primary culture of human nephroblasts. Proc. Natl. Acad. Sci. USA 90: 6066-6070, 1993. PubMed: 8392186 Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewings Sarcoma=Wilms Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614 Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007 |
| Cross References： | Nucleotide (GenBank) : D10232 Homo sapiens mRNA for renin-binding protein, complete cds. |
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| 产品名称： | G-401 G401 |
|---|---|
| 商品货号： | TS212129 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | rhabdoid tumor (formerly classified as Wilms tumor) |
| Age： | 3 months |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Karyotype： | The cell line has the 46, XY karyotype. There are no apparent marker chromosomes. The rate of polyploidy was 2.8%. The presence of the Y chromosome was confirmed in Q-band observation. |
| Images： | |
| Derivation： | G-401 was deposited as a cell line derived from a Wilms tumor. |
| Clinical Data： | 3 months Caucasian male |
| Genes Expressed： | nephroblast growth factor (NB-GF) |
| Cellular Products： | nephroblast growth factor (NB-GF) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms colonies in soft agar |
| Comments： | G-401 was deposited as a cell line derived from a Wilms tumor. Due to a change in the classification of such tumors, the cell line was examined by Garvin et al. and found to be more appropriately classified as derived from a rhabdoid tumor of the kidney. Ref Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 9,14 D16S539: 12 D5S818: 13 D7S820: 11,14 THO1: 8,9.3 TPOX: 8,11 vWA: 16 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | PT Peebles |
| Deposited As： | Homo sapiens |
| References： | Weissman BE, et al. Introduction of a normal human chromosome 11 into a Wilms tumor cell line controls its tumorigenic expression. Science 236: 175-176, 1987. PubMed: 3031816 Burrow CR, Wilson PD. A putative Wilms tumor-secreted growth factor activity required for primary culture of human nephroblasts. Proc. Natl. Acad. Sci. USA 90: 6066-6070, 1993. PubMed: 8392186 Karnieli E, et al. The IGF-1 receptor gene promoter is a molecular target for the Ewings Sarcoma=Wilms Tumor 1 fusion protein. J. Biol. Chem. 271: 19304-19309, 1996. PubMed: 8702614 Garvin AJ, et al. The G401 cell line, utilized for studies of chromosomal changes in Wilms tumor, is derived from a rhabdoid tumor of the kidney. Am. J. Pathol. 142: 375-380, 1993. PubMed: 8382007 |
| Cross References： | Nucleotide (GenBank) : D10232 Homo sapiens mRNA for renin-binding protein, complete cds. |
