G-8

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货期:

编号:TS212135

品牌:Testobio

产品名称：	G-8
商品货号：	TS212135
Organism：	Mus musculus, mouse
Tissue：	skeletal muscle
Cell Type：	myoblast myoblast
Product Format：	frozen
Morphology：	myoblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	fetus fetus
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 66
Receptor Expression：	acetylcholine (The cells are highly responsive to acetylcholine.)
Genes Expressed：	myosin
Cellular Products：	myosin
Tumorigenic：	No
Effects：	No, in immunosuppressed mice Yes, semi-solid media
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10% Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Subculturing：	The cells should be subcultured before they become confluent. Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new Bovine Collagen type l coated flasks. (0.03 mg/mL) Incubate cultures at 37°C. Myotubes form at confluency. Differentiation is improved by reducing the concentration of both sera to 2% each Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Population Doubling Time：	25 to 27 hrs
Name of Depositor：	J Peacock
Deposited As：	Mus musculus
References：	Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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G-8

货号: TS212135
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产品名称：	G-8
商品货号：	TS212135
Organism：	Mus musculus, mouse
Tissue：	skeletal muscle
Cell Type：	myoblast myoblast
Product Format：	frozen
Morphology：	myoblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Age：	fetus fetus
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	modal number = 66
Receptor Expression：	acetylcholine (The cells are highly responsive to acetylcholine.)
Genes Expressed：	myosin
Cellular Products：	myosin
Tumorigenic：	No
Effects：	No, in immunosuppressed mice Yes, semi-solid media
Complete Growth Medium：	Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10% Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Subculturing：	The cells should be subcultured before they become confluent. Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new Bovine Collagen type l coated flasks. (0.03 mg/mL) Incubate cultures at 37°C. Myotubes form at confluency. Differentiation is improved by reducing the concentration of both sera to 2% each Subcultivation Ratio: 1:2 to 1:4 Medium Renewal: Every 2 to 3 days
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Population Doubling Time：	25 to 27 hrs
Name of Depositor：	J Peacock
Deposited As：	Mus musculus
References：	Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.