| 产品名称： | FT |
|---|---|
| 商品货号： | TS212139 |
| Organism： | Rana catesbelana, bullfrog |
| Tissue： | tongue, normal |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 43; range = 36 to 49 Interesting extended centromeric region in chromosomes of many cells; chromosomes are large; some chromosomes have the secondary constrictions found in diploid cells. Karyotype of stemline cells unstable. |
| Clinical Data： | female |
| Virus Resistance： | poliovirus 1; herpes simplex; adenovirus 3; vesicular stomatitis (Indiana); infectious pancreatic necrosis virus of trout |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 10% double distilled water and 10% fetal bovine serum.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.xa0xa0 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 25°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | K Wolf |
| Deposited As： | Rana catesbelana |
| Year of Origin： | July, 1961 |
| References： | Wolf K, Quimby MC. Amphibian cell culture: permanent cell line from the bullfrog (Rana catesbeiana). Science 144: 1578-1580, 1964. PubMed: 14169344 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
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| 产品名称： | FT |
|---|---|
| 商品货号： | TS212139 |
| Organism： | Rana catesbelana, bullfrog |
| Tissue： | tongue, normal |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | adult |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 43; range = 36 to 49 Interesting extended centromeric region in chromosomes of many cells; chromosomes are large; some chromosomes have the secondary constrictions found in diploid cells. Karyotype of stemline cells unstable. |
| Clinical Data： | female |
| Virus Resistance： | poliovirus 1; herpes simplex; adenovirus 3; vesicular stomatitis (Indiana); infectious pancreatic necrosis virus of trout |
| Complete Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 10% double distilled water and 10% fetal bovine serum.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:3 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.xa0xa0 |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 25°C Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | K Wolf |
| Deposited As： | Rana catesbelana |
| Year of Origin： | July, 1961 |
| References： | Wolf K, Quimby MC. Amphibian cell culture: permanent cell line from the bullfrog (Rana catesbeiana). Science 144: 1578-1580, 1964. PubMed: 14169344 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
